Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604191747_z2xq TGTGTAGAGGCACCTGAAGAGAGTGCACTTTG(+ACTCAC)GTACCAGTCAGCCGGGTTCCTGTGTCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240604191745_jpr5 TGTCTTTATTCTAATGTCTTTGGTGTGGGAAG(+ACTCAC)GTACCTCATAGGCTGCCAAAAGAGAAATGCA NGG 10 8 17 8 24 0 100 5 10
    240604191744_9uh8 ATGTTTGCATTTCTCTTTTGGCAGCCTATGAG(+ACTCAC)GTACCTTCCCACACCAAAGACATTAGAATAA NGG 10 8 17 8 24 0 100 5 10
    240604191743_oklq TGGGTATGGATGGAGCCACACTCCTATGCAAG(+ACTCAC)GATCCATTTCCATGCAAGATTGTCAATTCTT NGG 10 8 17 8 24 0 100 5 10
    240604191742_nvul TCTGGAAGAATTGACAATCTTGCATGGAAATG(+ACTCAC)GATCCTTGCATAGGAGTGTGGCTCCATCCAT NGG 10 8 17 8 24 0 100 5 10
    240604191740_r2d0 TTTTTAAGATGCTTCTGGAGATTTAGAATCAG(+ACTCAC)GATCCAAAATAAGGCTGGGATTCAGCATCCA NGG 10 8 17 8 24 0 100 5 10
    240604191739_igbo AGTGGTGGATGCTGAATCCCAGCCTTATTTTG(+ACTCAC)GATCCTGATTCTAAATCTCCAGAAGCATCTT NGG 10 8 17 8 24 0 100 5 10
    240604191737_5foy TGAGCGTATCTGCTGTCCTTCTGCATGCCCAG(+ACTCAC)GTACCTCAGTTTTTTTCAACAGCAAGGAAAC NGG 10 8 17 8 24 0 100 5 10
    240604191735_ku47 CCCTAGTTTCCTTGCTGTTGAAAAAAACTGAG(+ACTCAC)GTACCTGGGCATGCAGAAGGACAGCAGATAC NGG 10 8 17 8 24 0 100 5 10
    240604191734_b24x TTCCTTTGGTTTAAGCCCGTTATAATTAAATG(+ACTCAC)GTACCAAAAGCTTTAAGAAGTGCTGTTCTAC NGG 10 8 17 8 24 0 100 5 10