Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604191831_2dj0 ACCACTGTCTTTCAGCAGGGAAGTCTCAGTGG(+ACTCAC)GTACCAAGTTCATCTGAAGTTTCCAGCCTTT NGG 10 8 17 8 24 0 100 5 10
    240604191829_wehe AAGAAGCCCGGCGGAGCTTTAGGTGGCCACAG(+ACTCAC)GTACCTGCTGGCAGTGCTGTCACATTTAAGG NGG 10 8 17 8 24 0 100 5 10
    240604191828_npb0 CCTTGCCTTAAATGTGACAGCACTGCCAGCAG(+ACTCAC)GTACCTGTGGCCACCTAAAGCTCCGCCGGGC NGG 10 8 17 8 24 0 100 5 10
    240604191827_ns4w ACTTGGCTGAGGAGACTCGGCTTAGGAAGCAG(+ACTCAC)GTACCTGACTTTTGAGAAAACCCACTAGCTT NGG 10 8 17 8 24 0 100 5 10
    240604191825_z9l8 AACAGAAGCTAGTGGGTTTTCTCAAAAGTCAG(+ACTCAC)GTACCTGCTTCCTAAGCCGAGTCTCCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240604191824_8bjn AGGAATCGATTCATAGTCCTGGGTATCAAAGG(+ACTCAC)GATCCAGTCACCAGGAGCTCCTCTCTTACCA NGG 10 8 17 8 24 0 100 5 10
    240604191822_7n95 ATCCCTGGTAAGAGAGGAGCTCCTGGTGACTG(+ACTCAC)GATCCCTTTGATACCCAGGACTATGAATCGA NGG 10 8 17 8 24 0 100 5 10
    240604191821_c2rh GGGGACAAGGTCACCTGAACTTAGCTCTCAAG(+ACTCAC)GATCCACACAAACTCAAAGCCTTAGTGGTAG NGG 10 8 17 8 24 0 100 5 10
    240604191819_vm8e GGCCACTACCACTAAGGCTTTGAGTTTGTGTG(+ACTCAC)GATCCTTGAGAGCTAAGTTCAGGTGACCTTG NGG 10 8 17 8 24 0 100 5 10
    240604191818_4qfc ACACAGCAATGGGAATAGCCACCGAGTTTAAG(+ACTCAC)GTACCAGGTGGCAACAAACCAATAATAACTT NGG 10 8 17 8 24 0 100 5 10