Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604191903_ceah GTAACAATTTGTGAAGGCTACGATTAGCTTAG(+ACTCAC)GATCCTAATAAATACTCTAAAAGGGCTGTTT NGG 10 8 17 8 24 0 100 5 10
    240604191900_n520 GGATTGCCCCACACCGTCCCCAGTCAGCCCTG(+ACTCAC)GATCCATGTGTCCTTTACTTGGGGAAAACCT NGG 10 8 17 8 24 0 100 5 10
    240604191859_x9sq GTAGAAGGTTTTCCCCAAGTAAAGGACACATG(+ACTCAC)GATCCAGGGCTGACTGGGGACGGTGTGGGGC NGG 10 8 17 8 24 0 100 5 10
    240604191857_svp0 TGGCCTGCTACAAACCTAGGGAGGGAGAGAGG(+ACTCAC)GATCCGGGCAAGGTTCGTATTGTGTCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240604191855_ji7k CCCAGCCAGGGACACAATACGAACCTTGCCCG(+ACTCAC)GATCCCTCTCTCCCTCCCTAGGTTTGTAGCA NGG 10 8 17 8 24 0 100 5 10
    240604191852_jv23 CAGCTGACAGTGGTGCACCTTTCACAGCCAGG(+ACTCAC)GATCCATGGGGCAGGGGACAGGTGCAGGCGC NGG 10 8 17 8 24 0 100 5 10
    240604191850_wyi8 CAGCAGCGCCTGCACCTGTCCCCTGCCCCATG(+ACTCAC)GATCCCTGGCTGTGAAAGGTGCACCACTGTC NGG 10 8 17 8 24 0 100 5 10
    240604191849_tsmn TTTCAGGAACAATGAATGGTAGGACTCTATAG(+ACTCAC)GTACCCATGTTTCTCTTACTAAACTGTTGTT NGG 10 8 17 8 24 0 100 5 10
    240604191847_pymv ACTACAACAACAGTTTAGTAAGAGAAACATGG(+ACTCAC)GTACCTATAGAGTCCTACCATTCATTGTTCC NGG 10 8 17 8 24 0 100 5 10
    240604191846_txka TAAATCCTGTCTACAAAAATTATGTCATACAG(+ACTCAC)GTACCATTTGTTCTTTCTCTTTCCAAAGAGT NGG 10 8 17 8 24 0 100 5 10