Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604191918_2u21 AATTGCTCCAAACTGACACATTGTAGGATTTG(+ACTCAC)GTACCCCGTGTCCTGTCCATTTAAATGCAAT NGG 10 8 17 8 24 0 100 5 10
    240604191916_4n4i TGCTCTTTATTCTCAGGGCCCGCACACATCCG(+ACTCAC)GTACCTTCATAAATGCAGTGTCACTGAAGAA NGG 10 8 17 8 24 0 100 5 10
    240604191915_vcrk TCCATTTCTTCAGTGACACTGCATTTATGAAG(+ACTCAC)GTACCGGATGTGTGCGGGCCCTGAGAATAAA NGG 10 8 17 8 24 0 100 5 10
    240604191913_epwy AGCACACTGCTATGTGTGGCCTTGATGCTGGG(+ACTCAC)GATCCTAACTCAGGTTCTCGTGCTTTACCGA NGG 10 8 17 8 24 0 100 5 10
    240604191912_aqnz CCCAGTCGGTAAAGCACGAGAACCTGAGTTAG(+ACTCAC)GATCCCCAGCATCAAGGCCACACATAGCAGT NGG 10 8 17 8 24 0 100 5 10
    240604191910_c3ys AGAAACCAGGGCCAGCGTTGGGGGTGCAGGAG(+ACTCAC)GTACCCAGAGAAGCAAAAACATTCAAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240604191909_g34i CTCTTTTCCTTTGAATGTTTTTGCTTCTCTGG(+ACTCAC)GTACCTCCTGCACCCCCAACGCTGGCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240604191906_135b TAAAATTCAGTATTTAAATATTAATTGCCAGG(+ACTCAC)GTACCAAATAAATTGTCACTTATAAATATTT NGG 10 8 17 8 24 0 100 5 10
    240604191905_acee ACAAGAAATATTTATAAGTGACAATTTATTTG(+ACTCAC)GTACCCTGGCAATTAATATTTAAATACTGAA NGG 10 8 17 8 24 0 100 5 10
    240604191904_7k94 TGTGAAAACAGCCCTTTTAGAGTATTTATTAG(+ACTCAC)GATCCTAAGCTAATCGTAGCCTTCACAAATT NGG 10 8 17 8 24 0 100 5 10