Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604192510_cils TGGATACAGATAGCACAATCCACTACCCAGTG(+ACTCAC)GATCCCTCAGCTGAAGGTTACTGATACCTAA NGG 10 8 17 8 24 0 100 5 10
    240604192507_hwbo GCCCCTGATCCAAGTCTCCAAAAGAGGCCAGG(+ACTCAC)GATCCTTCGAGCAAAGAGATAAATCTTATTC NGG 10 8 17 8 24 0 100 5 10
    240604192506_2mgu CACTTGAATAAGATTTATCTCTTTGCTCGAAG(+ACTCAC)GATCCCTGGCCTCTTTTGGAGACTTGGATCA NGG 10 8 17 8 24 0 100 5 10
    240604192505_x87w TGAGCCCAGCATGGGCGATGGTCATCCTCATG(+ACTCAC)GATCCAGGATAGATGGTCACTCTCAGCATGG NGG 10 8 17 8 24 0 100 5 10
    240604192503_afad CCCAGCCATGCTGAGAGTGACCATCTATCCTG(+ACTCAC)GATCCATGAGGATGACCATCGCCCATGCTGG NGG 10 8 17 8 24 0 100 5 10
    240604192500_ubwv GGAGATGTTTTTATAAAGCTGATGGCTCCTAG(+ACTCAC)GATCCCTGTGTTTGTCTACAGCTCAACACAG NGG 10 8 17 8 24 0 100 5 10
    240604192459_vvzs TACTACTGTGTTGAGCTGTAGACAAACACAGG(+ACTCAC)GATCCTAGGAGCCATCAGCTTTATAAAAACA NGG 10 8 17 8 24 0 100 5 10
    240604192457_du73 GATAGGGAGGGCATGCCTCGCTTTGGATGAGG(+ACTCAC)GATCCATTTAAACACTACCGTTTGGAATTGA NGG 10 8 17 8 24 0 100 5 10
    240604192456_j2l6 AGCCCTCAATTCCAAACGGTAGTGTTTAAATG(+ACTCAC)GATCCCTCATCCAAAGCGAGGCATGCCCTCC NGG 10 8 17 8 24 0 100 5 10
    240604192455_q32t ATCCTCTGCCCGCAATGCTTTTGAAAGACTTG(+ACTCAC)GATCCTTTTGAAATGGATGCTGAGCTGCACT NGG 10 8 17 8 24 0 100 5 10