Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604192039_9lqh AGGTCCCAACAGGCAGGGGAGGAACTTCTGAG(+ACTCAC)GTACCAACTTCATAGCCCTACTGTGTATAGA NGG 10 8 17 8 24 0 100 5 10
    240604192037_q4if GCTCCGAGGTCAGGTGGACTGTCAGGGCCTAG(+ACTCAC)GATCCACCAGTTTGCCCGAGTTTACATTAGC NGG 10 8 17 8 24 0 100 5 10
    240604192036_icr0 ATGTTGCTAATGTAAACTCGGGCAAACTGGTG(+ACTCAC)GATCCTAGGCCCTGACAGTCCACCTGACCTC NGG 10 8 17 8 24 0 100 5 10
    240604192033_gqk2 CTCCTGAGCCAATAGGGGCTTCCAGACCCAAG(+ACTCAC)GATCCGTTTCCTTGATGACTAAGAAGCCCCA NGG 10 8 17 8 24 0 100 5 10
    240604192032_79cx AAGGGTGGGGCTTCTTAGTCATCAAGGAAACG(+ACTCAC)GATCCTTGGGTCTGGAAGCCCCTATTGGCTC NGG 10 8 17 8 24 0 100 5 10
    240604192030_2e6m TCCAAGAGCCCTGCTCTGCCCCTCGTGGCATG(+ACTCAC)GATCCTTTCCCTGGTGTGGACTTAAGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240604192029_ajhv TCCCCAGAGACTTAAGTCCACACCAGGGAAAG(+ACTCAC)GATCCATGCCACGAGGGGCAGAGCAGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240604192028_s3zw CAGGAGGCTGCTATGGGCTAAAATTTTCCTAG(+ACTCAC)GTACCACCTAGAGGAGATGGAGCCGACCGAG NGG 10 8 17 8 24 0 100 5 10
    240604192026_oa27 CTTCCCTCGGTCGGCTCCATCTCCTCTAGGTG(+ACTCAC)GTACCTAGGAAAATTTTAGCCCATAGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240604192025_wlvf CAGGAATGCCATCTTCTGCCTTGGTCTGGAAG(+ACTCAC)GTACCCATGGCTACTTGCATCATTTTCCAGC NGG 10 8 17 8 24 0 100 5 10