Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604192111_56nc GATCTCCCATCTCTTTGTGTCGCTGAGGCAAG(+ACTCAC)GTACCTTTGGTTTCAAAAGTGGGTTAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240604192109_g4p5 TCTCTGTCCTGGTCCCGCCCCCAACCATGGTG(+ACTCAC)GATCCTGGAGGACCTCGTACCCCTCCCCTCC NGG 10 8 17 8 24 0 100 5 10
    240604192107_rj1i GTGGGGGAGGGGAGGGGTACGAGGTCCTCCAG(+ACTCAC)GATCCACCATGGTTGGGGGCGGGACCAGGAC NGG 10 8 17 8 24 0 100 5 10
    240604192105_ydon CTGCCTATAAATACCTCTCCAGGGGCTGGGAG(+ACTCAC)GTACCCTCTGTTCCATCCACCACACTGCTCA NGG 10 8 17 8 24 0 100 5 10
    240604192104_noj8 GGCAATGAGCAGTGTGGTGGATGGAACAGAGG(+ACTCAC)GTACCTCCCAGCCCCTGGAGAGGTATTTATA NGG 10 8 17 8 24 0 100 5 10
    240604192102_es8j ATCCAAATGCCTAGTACAGGGAAGCCGTAGAG(+ACTCAC)GTACCATCTGCAGCTTATTTTAAAAGCCATC NGG 10 8 17 8 24 0 100 5 10
    240604192101_08dq CCACTGATGGCTTTTAAAATAAGCTGCAGATG(+ACTCAC)GTACCTCTACGGCTTCCCTGTACTAGGCATT NGG 10 8 17 8 24 0 100 5 10
    240604192059_7qik TGAGCGGCTGCCGCCCCACCCTGTGCTCTCCG(+ACTCAC)GATCCCACTAGCCACAGGCATCTCCAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240604192058_gfpa CGGGCCCTCCTGGAGATGCCTGTGGCTAGTGG(+ACTCAC)GATCCGGAGAGCACAGGGTGGGGCGGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240604192056_vrpj GCACCCAGATAGGCAAGGTCACCTTGACACTG(+ACTCAC)GTACCATGGTAACCTTGGCTCTTTAGGGCTG NGG 10 8 17 8 24 0 100 5 10