Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604191950_b9zh CTGGGGATGGCATGGACTTAGTGGGCACTGAG(+ACTCAC)GATCCTCCTGGCAGGAGTTCTGAACACCCAA NGG 10 8 17 8 24 0 100 5 10
    240604191949_9t3c TTCAATAAATACTTGTTGCATGAACAAACTTG(+ACTCAC)GATCCAAATTTTTAGAAATATTGAATTTGGA NGG 10 8 17 8 24 0 100 5 10
    240604191947_x1b3 GAGCATCCAAATTCAATATTTCTAAAAATTTG(+ACTCAC)GATCCAAGTTTGTTCATGCAACAAGTATTTA NGG 10 8 17 8 24 0 100 5 10
    240604191946_gone GTGTCCATGTTTAAAGACATCATCCCTGCCAG(+ACTCAC)GATCCATGAGAAAAAATATATTTTCTTACTC NGG 10 8 17 8 24 0 100 5 10
    240604191944_ljij TGTGGGAGTAAGAAAATATATTTTTTCTCATG(+ACTCAC)GATCCTGGCAGGGATGATGTCTTTAAACATG NGG 10 8 17 8 24 0 100 5 10
    240604191943_gq69 AGAGCAGCCTCAGAGAGGCTTGTCCTAGTCAG(+ACTCAC)GATCCAGGATCTTGGCTGGGGCCTGCTTCTT NGG 10 8 17 8 24 0 100 5 10
    240604191941_n33j GAACAAAGAAGCAGGCCCCAGCCAAGATCCTG(+ACTCAC)GATCCTGACTAGGACAAGCCTCTCTGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240604191940_d2ik CCAGGCATGCTCCTGCCTAGGTGAAATGAAAG(+ACTCAC)GATCCACATACTGGCACATGACATGATCCTA NGG 10 8 17 8 24 0 100 5 10
    240604191939_5urq CTGAATAGGATCATGTCATGTGCCAGTATGTG(+ACTCAC)GATCCTTTCATTTCACCTAGGCAGGAGCATG NGG 10 8 17 8 24 0 100 5 10
    240604191937_z8r9 CCTTTTAGCTGCCATCTGCCAAGCACACCTGG(+ACTCAC)GATCCACCAGTTCTGGCAATGCTGTGACAGT NGG 10 8 17 8 24 0 100 5 10