Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604192437_5rte AGTGTGTATTCCCCGTCCCCTCTGACACCTGG(+ACTCAC)GATCCAGATTTTGAATTGTGGCCCTGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240604192436_3iia TTAAAAGAGTAGCTTCCTCTGCTGTACTAGTG(+ACTCAC)GATCCTGTTTTTGTTGCTTCCAGGGACCAGC NGG 10 8 17 8 24 0 100 5 10
    240604192434_rmuv AAAAAGCTGGTCCCTGGAAGCAACAAAAACAG(+ACTCAC)GATCCACTAGTACAGCAGAGGAAGCTACTCT NGG 10 8 17 8 24 0 100 5 10
    240604192432_h042 TTTACATGAAGTGATGAGACCAAAGGCCCAAG(+ACTCAC)GATCCCCGTCCCCACCCCCCACCCCCTTTTG NGG 10 8 17 8 24 0 100 5 10
    240604192430_a085 GAAAACAAAAGGGGGTGGGGGGTGGGGACGGG(+ACTCAC)GATCCTTGGGCCTTTGGTCTCATCACTTCAT NGG 10 8 17 8 24 0 100 5 10
    240604192428_s1rt CAGAGCCAGTTTGGTCTTTCTCCCCCTGCCAG(+ACTCAC)GATCCCAGGCCCTAATCAGTATTATTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240604192426_m593 TTATGAAAGAAATAATACTGATTAGGGCCTGG(+ACTCAC)GATCCTGGCAGGGGGAGAAAGACCAAACTGG NGG 10 8 17 8 24 0 100 5 10
    240604192425_gaei AGCTTCAGGGTCCTCCTCGCTGTCCACACATG(+ACTCAC)GATCCCAGCGGAATCTCCGCTGACACGGAAG NGG 10 8 17 8 24 0 100 5 10
    240604192423_fvro TTTGCCTTCCGTGTCAGCGGAGATTCCGCTGG(+ACTCAC)GATCCATGTGTGGACAGCGAGGAGGACCCTG NGG 10 8 17 8 24 0 100 5 10
    240604192421_h0zo TAGGCCCCTCCCAGCTGTGAGGGTCCAGGGAG(+ACTCAC)GATCCTGTGGCCGAGTCTTCAGTGGCCGAGT NGG 10 8 17 8 24 0 100 5 10