Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604191626_l8ea TTCAGGGAAGCCTGGGTCTTGGTTGGAGATTG(+ACTCAC)GTACCTACCACTGAGACACCTCTCACTTCCG NGG 10 8 17 8 24 0 100 5 10
    240604191623_d1z0 GCAGGCAGCAAGGAGACCTGAGGTGGCCCTGG(+ACTCAC)GTACCAGGACCCCAGAGTAGAGGTGAGGATG NGG 10 8 17 8 24 0 100 5 10
    240604191621_0psg CTGTGCATCCTCACCTCTACTCTGGGGTCCTG(+ACTCAC)GTACCCAGGGCCACCTCAGGTCTCCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240604191620_xqqy CCACCAAGTGCACAGCACCCACCCCGTATGTG(+ACTCAC)GTACCCTGTACGCGTGGATCTTTGTGTGCAC NGG 10 8 17 8 24 0 100 5 10
    240604191618_i6m3 GATCTGTGCACACAAAGATCCACGCGTACAGG(+ACTCAC)GTACCACATACGGGGTGGGTGCTGTGCACTT NGG 10 8 17 8 24 0 100 5 10
    240604191617_r9rz CCTCTGGGACGTCTGTGATGCTCCTTCTCTGG(+ACTCAC)GTACCTGAAGAGGCTCCTCCACGACCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240604191616_omsb GAGATGGCTTGGTCGTGGAGGAGCCTCTTCAG(+ACTCAC)GTACCCAGAGAAGGAGCATCACAGACGTCCC NGG 10 8 17 8 24 0 100 5 10
    240604191614_3b5c AAGCTCACCCCATCCCTGTGCGGTTGCCTGAG(+ACTCAC)GTACCTTCACGAGGAGAGATACACTGGCCCG NGG 10 8 17 8 24 0 100 5 10
    240604191612_n4t1 GCGGACGGGCCAGTGTATCTCTCCTCGTGAAG(+ACTCAC)GTACCTCAGGCAACCGCACAGGGATGGGGTG NGG 10 8 17 8 24 0 100 5 10
    240604191611_10a8 CAGCAACATCTTCTTCTCAGGGCCACACACTG(+ACTCAC)GATCCTGGGAGAGATGCCCACTTAAGCTTCC NGG 10 8 17 8 24 0 100 5 10