Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604191553_iddz TACCAATGGTTGTCACATCAAAGGGAGCCCTG(+ACTCAC)GATCCGAAGCTGGAGAGGCTAGCATGTGACT NGG 10 8 17 8 24 0 100 5 10
    240604191551_hvjp GTGCCCGGTGTGCAGGCACCAAGGAGACACAG(+ACTCAC)GTACCAGCAGACCACCACTGGTGACATCATC NGG 10 8 17 8 24 0 100 5 10
    240604191550_qgfu CTGGTGATGATGTCACCAGTGGTGGTCTGCTG(+ACTCAC)GTACCTGTGTCTCCTTGGTGCCTGCACACCG NGG 10 8 17 8 24 0 100 5 10
    240604191548_13pm AGTCCTCACCGAAGTCACATCAACTTCCTATG(+ACTCAC)GTACCTCTCATGAACCTGGGGTGCTTCTTAC NGG 10 8 17 8 24 0 100 5 10
    240604191547_884h ACGGGGTAAGAAGCACCCCAGGTTCATGAGAG(+ACTCAC)GTACCATAGGAAGTTGATGTGACTTCGGTGA NGG 10 8 17 8 24 0 100 5 10
    240604191546_nybz AGTGCTTGTCTTACATGCACAGGATCTGGTTG(+ACTCAC)GATCCCCATGCATCATTGACACTGGGTATTG NGG 10 8 17 8 24 0 100 5 10
    240604191544_wc5k ACACCCAATACCCAGTGTCAATGATGCATGGG(+ACTCAC)GATCCAACCAGATCCTGTGCATGTAAGACAA NGG 10 8 17 8 24 0 100 5 10
    240604191542_t7pu CAGCAGGCAGAGGTGAAAAGAACACTGCTGTG(+ACTCAC)GATCCCAATCAAAAGTGTTTTACATGCAAAG NGG 10 8 17 8 24 0 100 5 10
    240604191541_bz1s TATGGCTTTGCATGTAAAACACTTTTGATTGG(+ACTCAC)GATCCACAGCAGTGTTCTTTTCACCTCTGCC NGG 10 8 17 8 24 0 100 5 10
    240604191539_5sik CCAGGCCAGCACAGTGCTTCCTGGTAGGGTGG(+ACTCAC)GTACCAGCCATACTGAGAATCGGGCCAGGCC NGG 10 8 17 8 24 0 100 5 10