Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604190931_wchc TCCCAAACTTAGGGCTTCCTCCATGTGCAAGG(+ACTCAC)GTACCGTCACTGAGACACATCGCTAGCACAA NGG 10 8 17 8 24 0 100 5 10
    240604190930_2v6s TAAGCTTGTGCTAGCGATGTGTCTCAGTGACG(+ACTCAC)GTACCCTTGCACATGGAGGAAGCCCTAAGTT NGG 10 8 17 8 24 0 100 5 10
    240604190927_9jze CTCGAATCCAAAACTGTATAGACGTTCCAAAG(+ACTCAC)GTACCTGTTTTCTAAGCTCTAATCCTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240604190926_ji68 GAAAGAGAAGAGGATTAGAGCTTAGAAAACAG(+ACTCAC)GTACCTTTGGAACGTCTATACAGTTTTGGAT NGG 10 8 17 8 24 0 100 5 10
    240604190924_v936 ACAAGACAACACCACCACGGAAGCAGAGGGGG(+ACTCAC)GATCCATGCACAGCCTTTTCAGGGTCTCTTG NGG 10 8 17 8 24 0 100 5 10
    240604190922_h3df AGCCCCAAGAGACCCTGAAAAGGCTGTGCATG(+ACTCAC)GATCCCCCCTCTGCTTCCGTGGTGGTGTTGT NGG 10 8 17 8 24 0 100 5 10
    240604190921_hhv2 TCACATCTCCCTTCAGAAATCATGTTTGCAGG(+ACTCAC)GATCCGGTTTTGTATTTTGTTTTTAAACAAG NGG 10 8 17 8 24 0 100 5 10
    240604190919_sm6w TTAGACTTGTTTAAAAACAAAATACAAAACCG(+ACTCAC)GATCCCTGCAAACATGATTTCTGAAGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240604190918_jx2s GGACCTTTATTTACCTAACTTAAGATCATTAG(+ACTCAC)GTACCTGGTTTGGCTTACACTCAAACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240604190916_y1dp GCAGACCTGGGTTTGAGTGTAAGCCAAACCAG(+ACTCAC)GTACCTAATGATCTTAAGTTAGGTAAATAAA NGG 10 8 17 8 24 0 100 5 10