Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604191004_q0a7 TTAGACCTAATAAAAGCCGAGGGTGAAATCTG(+ACTCAC)GATCCCTGTTTAGCACACCTGAGTTGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240604191002_pc7s TGGGGACAGACAACTCAGGTGTGCTAAACAGG(+ACTCAC)GATCCAGATTTCACCCTCGGCTTTTATTAGG NGG 10 8 17 8 24 0 100 5 10
    240604191001_n4nc TTGAAGCCTGCAGGACCCTTCAACCAACTATG(+ACTCAC)GATCCCTTCGCGCTTCCCTGCTGGCTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240604190959_8syt ACTGAACAAGAGCCAGCAGGGAAGCGCGAAGG(+ACTCAC)GATCCATAGTTGGTTGAAGGGTCCTGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240604190958_4bfq ATCCCTTCCCTCACTCACCCTCTGAGATCATG(+ACTCAC)GTACCCACTTGTTAAATGCATACTTCAAAAT NGG 10 8 17 8 24 0 100 5 10
    240604190956_1we8 ATGGCATTTTGAAGTATGCATTTAACAAGTGG(+ACTCAC)GTACCATGATCTCAGAGGGTGAGTGAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240604190954_k86h ATTTAAGACATTACCTCCTAGCATTCCAAGGG(+ACTCAC)GATCCCAGATTTGAAAGGGCAAGCCTGGCAT NGG 10 8 17 8 24 0 100 5 10
    240604190952_jltr CATTTATGCCAGGCTTGCCCTTTCAAATCTGG(+ACTCAC)GATCCCCTTGGAATGCTAGGAGGTAATGTCT NGG 10 8 17 8 24 0 100 5 10
    240604190951_e1w2 TTCATTTTAGATTACTAATAAAGGGAGAAAAG(+ACTCAC)GTACCTTCAAAGCTTATTCACATTTGCAAAA NGG 10 8 17 8 24 0 100 5 10
    240604190949_ddoq AAAGATTTTGCAAATGTGAATAAGCTTTGAAG(+ACTCAC)GTACCTTTTCTCCCTTTATTAGTAATCTAAA NGG 10 8 17 8 24 0 100 5 10