Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604191511_r98d TCTCCAGTCTGGGCACAGCCGGAACTGACCGG(+ACTCAC)GATCCAGAAATGTCACAGATGCGCAAGCCGC NGG 10 8 17 8 24 0 100 5 10
    240604191509_gxjw GTGGTTGCAACTTCTGGAGCAGGTGGGTGTGG(+ACTCAC)GATCCCAGGTGTAGTGTTGGCTGAGCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240604191507_dldo TGTCATCTGAGCTCAGCCAACACTACACCTGG(+ACTCAC)GATCCCACACCCACCTGCTCCAGAAGTTGCA NGG 10 8 17 8 24 0 100 5 10
    240604191505_10hl AGAGTCCCAACTCCAGTGAGCAGTAGGGCAGG(+ACTCAC)GATCCCACTCAGATCACAAAGTCATCATGGT NGG 10 8 17 8 24 0 100 5 10
    240604191503_rp6f CGCGGACCATGATGACTTTGTGATCTGAGTGG(+ACTCAC)GATCCCTGCCCTACTGCTCACTGGAGTTGGG NGG 10 8 17 8 24 0 100 5 10
    240604191501_ic6a TGGCAGTCATAGTGCCTAGAGCTTCTTCCCTG(+ACTCAC)GTACCCAGGAGTTCCTCACCACTCCCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240604191459_mful TAACTTCCAGGGGAGTGGTGAGGAACTCCTGG(+ACTCAC)GTACCAGGGAAGAAGCTCTAGGCACTATGAC NGG 10 8 17 8 24 0 100 5 10
    240604191457_7tmt AGCTGTCTGCCCTTCATGCTCCAGGGCAGCTG(+ACTCAC)GTACCGTGGAAAGTGCCTGGGGTGGTTTTGC NGG 10 8 17 8 24 0 100 5 10
    240604191456_rhw5 TATTTGCAAAACCACCCCAGGCACTTTCCACG(+ACTCAC)GTACCAGCTGCCCTGGAGCATGAAGGGCAGA NGG 10 8 17 8 24 0 100 5 10
    240604191455_f522 TACAGAGTAAGAGCCTGTCTCAAAAAAAAAAG(+ACTCAC)GTACCATTTTTATTGTGAGGGCTCCATGTAG NGG 10 8 17 8 24 0 100 5 10