Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604191056_qq5o TGTAAAATTCCAGTGACTCAAATGGCTTTAGG(+ACTCAC)GATCCCCTGGAGACGGTTCAGGAAGGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240604191053_7i5z CCAACAACTCCCTTCCTGAACCGTCTCCAGGG(+ACTCAC)GATCCCTAAAGCCATTTGAGTCACTGGAATT NGG 10 8 17 8 24 0 100 5 10
    240604191051_md9s GCAAAACTAACCTAAGGCTAAGTGACCACAGG(+ACTCAC)GATCCTCTGGGTGAACAGTGAGGACTATAAC NGG 10 8 17 8 24 0 100 5 10
    240604191048_6hfs CTGTGGTTATAGTCCTCACTGTTCACCCAGAG(+ACTCAC)GATCCCTGTGGTCACTTAGCCTTAGGTTAGT NGG 10 8 17 8 24 0 100 5 10
    240604191046_qoal CTCGGTGCATAACCTTGATCGTGGAGCCTCAG(+ACTCAC)GATCCCAAAACAGGATGTTTTGGGTGTCAAT NGG 10 8 17 8 24 0 100 5 10
    240604191044_gxl5 ATCAGATTGACACCCAAAACATCCTGTTTTGG(+ACTCAC)GATCCTGAGGCTCCACGATCAAGGTTATGCA NGG 10 8 17 8 24 0 100 5 10
    240604191042_rd9l TGGAAGAGTTGTGTCCTTCCCCTACGAAGGTG(+ACTCAC)GTACCGATGTGCTCTGCCTTTCTGATCCCAT NGG 10 8 17 8 24 0 100 5 10
    240604191041_tvye ATAAAATGGGATCAGAAAGGCAGAGCACATCG(+ACTCAC)GTACCACCTTCGTAGGGGAAGGACACAACTC NGG 10 8 17 8 24 0 100 5 10
    240604191039_56s5 CACACACGTGCATGCTCTGTGCGTGTATGTGG(+ACTCAC)GTACCGTGTATATGTGTGTGAGTGTTGTATA NGG 10 8 17 8 24 0 100 5 10
    240604191037_k0q9 ATGCATATACAACACTCACACACATATACACG(+ACTCAC)GTACCCACATACACGCACAGAGCATGCACGT NGG 10 8 17 8 24 0 100 5 10