Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604191200_50sc ATAGGTGCTGGAGAAGCGGGGACCGCAGTGGG(+ACTCAC)GTACCATCAGGCTCGGGGTAAGGTAGAAAAG NGG 10 8 17 8 24 0 100 5 10
    240604191159_bqd2 TTACCCTTTTCTACCTTACCCCGAGCCTGATG(+ACTCAC)GTACCCCACTGCGGTCCCCGCTTCTCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240604191158_grhy CTCCTCTACACAGCAGCAACTAAAATGATCAG(+ACTCAC)GATCCTTCACTGGAGCCCTAGAGACTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240604191157_wemg ATCTTCTGGGAGTCTCTAGGGCTCCAGTGAAG(+ACTCAC)GATCCTGATCATTTTAGTTGCTGCTGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240604191155_xlt1 CATACCTAGGAGCAAGCACTGATCGCTGAGCG(+ACTCAC)GTACCAAGAGGAGCCTCTCCTTTTACTGGAT NGG 10 8 17 8 24 0 100 5 10
    240604191154_ddpp TCTCCATCCAGTAAAAGGAGAGGCTCCTCTTG(+ACTCAC)GTACCGCTCAGCGATCAGTGCTTGCTCCTAG NGG 10 8 17 8 24 0 100 5 10
    240604191153_bfmr GGCCCCATCATTTGTAGATGTAGATCTTGTAG(+ACTCAC)GTACCTGCTATGGCAACTCATTTTATTCTCA NGG 10 8 17 8 24 0 100 5 10
    240604191152_ku2g GTACTTGAGAATAAAATGAGTTGCCATAGCAG(+ACTCAC)GTACCTACAAGATCTACATCTACAAATGATG NGG 10 8 17 8 24 0 100 5 10
    240604191151_0da1 ACAGAAGATTACCAGTTTAACAGACTTGTTTG(+ACTCAC)GATCCCTGTCCTATGGTTCCTAGGTTGTCAT NGG 10 8 17 8 24 0 100 5 10
    240604191149_edg1 CTCAGATGACAACCTAGGAACCATAGGACAGG(+ACTCAC)GATCCAAACAAGTCTGTTAAACTGGTAATCT NGG 10 8 17 8 24 0 100 5 10