Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604191231_nxds AGAGCAGATTAGGACACCACCTGACAGAACAG(+ACTCAC)GTACCTTGATGAGGTTTATCAGTAACTCGGT NGG 10 8 17 8 24 0 100 5 10
    240604191230_18px TGTCCACACATCCCTCTCTAAGCCCAGGATAG(+ACTCAC)GTACCAATATAGGTAACTCTGCATTTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240604191229_ms8v CTTATGGGAAAAATGCAGAGTTACCTATATTG(+ACTCAC)GTACCTATCCTGGGCTTAGAGAGGGATGTGT NGG 10 8 17 8 24 0 100 5 10
    240604191227_cank TACACCTCTAGGAACTGAGGCAGCTTCAGAAG(+ACTCAC)GTACCTGCCAGATGTTGCTGTGGGAAGCATG NGG 10 8 17 8 24 0 100 5 10
    240604191226_llcb AAGGCCATGCTTCCCACAGCAACATCTGGCAG(+ACTCAC)GTACCTTCTGAAGCTGCCTCAGTTCCTAGAG NGG 10 8 17 8 24 0 100 5 10
    240604191223_4zh4 GATGGCTTACAACTGTAACCCTGGTTCCAGGG(+ACTCAC)GATCCAGTACCTTCTGACCTGCTCGGGTGCC NGG 10 8 17 8 24 0 100 5 10
    240604191222_seut TTCCTGGCACCCGAGCAGGTCAGAAGGTACTG(+ACTCAC)GATCCCCTGGAACCAGGGTTACAGTTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240604191220_rddo TTCCTTGAAGTGAGGTCAAGGTCATGTGGCCG(+ACTCAC)GATCCTATACTCACCACTGGCTGTCTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240604191219_xuck CTTGAGAGAAAGACAGCCAGTGGTGAGTATAG(+ACTCAC)GATCCGGCCACATGACCTTGACCTCACTTCA NGG 10 8 17 8 24 0 100 5 10
    240604191218_iucr TTATCCACAGGACCTGGGTGGAGCAGGATATG(+ACTCAC)GATCCCTCATACTGAAAGATGTAGGGAAAAG NGG 10 8 17 8 24 0 100 5 10