Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604191331_np63 TTCAGTCTGGGGAGCCAACCACATGAAGCTTG(+ACTCAC)GATCCCTGGTGGCTTCTGCCGGAAGGAAACA NGG 10 8 17 8 24 0 100 5 10
    240604191330_lazi TTCAAGTCACAAAGTTACTGTCCCACGGAAAG(+ACTCAC)GATCCCAATCTCCTGCAGGGACATTATGAGT NGG 10 8 17 8 24 0 100 5 10
    240604191328_o01h ACCTTACTCATAATGTCCCTGCAGGAGATTGG(+ACTCAC)GATCCTTTCCGTGGGACAGTAACTTTGTGAC NGG 10 8 17 8 24 0 100 5 10
    240604191327_38uw CCTTGACCATTATAATTTAAATGTGTTTGGAG(+ACTCAC)GTACCAGGCTCATGGGCATAATTTATAATAT NGG 10 8 17 8 24 0 100 5 10
    240604191325_uwq5 AGTTGATATTATAAATTATGCCCATGAGCCTG(+ACTCAC)GTACCTCCAAACACATTTAAATTATAATGGT NGG 10 8 17 8 24 0 100 5 10
    240604191323_xcj3 TGAATCATTAGAACGCTAGAAGACAATATGGG(+ACTCAC)GTACCCCTTTACCAAGATTTTGAGAAAGAAT NGG 10 8 17 8 24 0 100 5 10
    240604191321_wl1r CAGTCATTCTTTCTCAAAATCTTGGTAAAGGG(+ACTCAC)GTACCCCATATTGTCTTCTAGCGTTCTAATG NGG 10 8 17 8 24 0 100 5 10
    240604191320_zl7x AGGGATCCTATGACTGACCAACAAAAGTACAG(+ACTCAC)GTACCACCAGAGTCCAACTTGTTGAAACAAT NGG 10 8 17 8 24 0 100 5 10
    240604191318_s36p AATGCATTGTTTCAACAAGTTGGACTCTGGTG(+ACTCAC)GTACCTGTACTTTTGTTGGTCAGTCATAGGA NGG 10 8 17 8 24 0 100 5 10
    240604191317_ldyl TAATTTCTCATTACCCTCTGTAAACACAATGG(+ACTCAC)GTACCCTTTGTTTTCCTCTCCTAAAAATAAC NGG 10 8 17 8 24 0 100 5 10