Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604191343_f7fc GAAAGCACAAATCCTAAGCACAGCCTTTGAAG(+ACTCAC)GATCCTGCTAATTGTATGTCTTAACAATAAA NGG 10 8 17 8 24 0 100 5 10
    240604191342_405n AAGATTTTATTGTTAAGACATACAATTAGCAG(+ACTCAC)GATCCTTCAAAGGCTGTGCTTAGGATTTGTG NGG 10 8 17 8 24 0 100 5 10
    240604191341_6fy8 ACAAGTACAGTTTCCAGTGCCTCCCTGCTGTG(+ACTCAC)GTACCTGAAAAGTCTGAATGCAAGTAGTATG NGG 10 8 17 8 24 0 100 5 10
    240604191339_olr5 ATAGTCATACTACTTGCATTCAGACTTTTCAG(+ACTCAC)GTACCACAGCAGGGAGGCACTGGAAACTGTA NGG 10 8 17 8 24 0 100 5 10
    240604191337_kreo AAAAATGTTCCTGCAAAGGACATCCACTCCTG(+ACTCAC)GATCCTGATGCCATTCACACAATGAGATAGT NGG 10 8 17 8 24 0 100 5 10
    240604191335_cbds GCAGCACTATCTCATTGTGTGAATGGCATCAG(+ACTCAC)GATCCAGGAGTGGATGTCCTTTGCAGGAACA NGG 10 8 17 8 24 0 100 5 10
    240604191333_mdzs TGGGGTGTTTCCTTCCGGCAGAAGCCACCAGG(+ACTCAC)GATCCAAGCTTCATGTGGTTGGCTCCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240604191331_np63 TTCAGTCTGGGGAGCCAACCACATGAAGCTTG(+ACTCAC)GATCCCTGGTGGCTTCTGCCGGAAGGAAACA NGG 10 8 17 8 24 0 100 5 10
    240604191330_lazi TTCAAGTCACAAAGTTACTGTCCCACGGAAAG(+ACTCAC)GATCCCAATCTCCTGCAGGGACATTATGAGT NGG 10 8 17 8 24 0 100 5 10
    240604191328_o01h ACCTTACTCATAATGTCCCTGCAGGAGATTGG(+ACTCAC)GATCCTTTCCGTGGGACAGTAACTTTGTGAC NGG 10 8 17 8 24 0 100 5 10