Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604191259_8pej ACGTCTAAAGAAAAAAATATAAACCTGGCCTG(+ACTCAC)GTACCCATTACATATATACATAATATGTTAT NGG 10 8 17 8 24 0 100 5 10
    240604191258_xuke GCTCACAACCATGCATAACTCAAGTTTTAAGG(+ACTCAC)GATCCAGTGCTGACTTCTGGCCTCTGAGGGT NGG 10 8 17 8 24 0 100 5 10
    240604191256_k96f GCAGTACCCTCAGAGGCCAGAAGTCAGCACTG(+ACTCAC)GATCCCTTAAAACTTGAGTTATGCATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240604191255_m39l TTGGGGGAGATGCAGCACACACCCCAGATAGG(+ACTCAC)GATCCTATGACTGACCAACAAAAGTACAGGT NGG 10 8 17 8 24 0 100 5 10
    240604191253_iuka GTGGTACCTGTACTTTTGTTGGTCAGTCATAG(+ACTCAC)GATCCCTATCTGGGGTGTGTGCTGCATCTCC NGG 10 8 17 8 24 0 100 5 10
    240604191252_klzr CATGCCATTTTAGGGCCATTGTGATTCTCAGG(+ACTCAC)GATCCTAGGTAAAAATCCTGAATCTTTTGGG NGG 10 8 17 8 24 0 100 5 10
    240604191250_e75z TCTTACCCAAAAGATTCAGGATTTTTACCTAG(+ACTCAC)GATCCCTGAGAATCACAATGGCCCTAAAATG NGG 10 8 17 8 24 0 100 5 10
    240604191248_6ivx TCTTGCTTGTCAACTTCAGAGATAGCTGGAAG(+ACTCAC)GATCCTGAGACTGTATGGCAGGAGAGTTGAG NGG 10 8 17 8 24 0 100 5 10
    240604191247_4oe1 AACCCCTCAACTCTCCTGCCATACAGTCTCAG(+ACTCAC)GATCCTTCCAGCTATCTCTGAAGTTGACAAG NGG 10 8 17 8 24 0 100 5 10
    240604191246_9mh5 AAAAGCATGAGATTTGCATCCCAGCTGTCCTG(+ACTCAC)GATCCAGGCTGTGAGGTTTTGAGGATTCTTA NGG 10 8 17 8 24 0 100 5 10