Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604191216_kgp7 ACTTCCTTTTCCCTACATCTTTCAGTATGAGG(+ACTCAC)GATCCATATCCTGCTCCACCCAGGTCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240604191214_kxus GGGGTACGTTATCTACTGCCTTCTCCCTTCTG(+ACTCAC)GATCCACGATAAAGCTCTGGCTTTAATAAAC NGG 10 8 17 8 24 0 100 5 10
    240604191213_id4i AACCAGTTTATTAAAGCCAGAGCTTTATCGTG(+ACTCAC)GATCCAGAAGGGAGAAGGCAGTAGATAACGT NGG 10 8 17 8 24 0 100 5 10
    240604191211_le5w CCCTGCGGTGGGAGGGGCAGGCAGGGGAGGTG(+ACTCAC)GTACCCTCCCACCAACCTGAGCCCACTCTCT NGG 10 8 17 8 24 0 100 5 10
    240604191210_spe3 TCTGAACAAAAGGAAGGAAAATGATTGGACAG(+ACTCAC)GTACCCCACAAACTTTCCATCTAAGTGTTGC NGG 10 8 17 8 24 0 100 5 10
    240604191208_wh84 GTTGGGCAACACTTAGATGGAAAGTTTGTGGG(+ACTCAC)GTACCTGTCCAATCATTTTCCTTCCTTTTGT NGG 10 8 17 8 24 0 100 5 10
    240604191207_gop6 CCCTAGCCTAGGGCTTCGACATTGCATGCTGG(+ACTCAC)GATCCAGCTCCTACCCTTCCTGCACTCCCTT NGG 10 8 17 8 24 0 100 5 10
    240604191205_g6vs TGGGGAAGGGAGTGCAGGAAGGGTAGGAGCTG(+ACTCAC)GATCCCAGCATGCAATGTCGAAGCCCTAGGC NGG 10 8 17 8 24 0 100 5 10
    240604191204_6ln4 CTAACCACTGCCCTCCTGGAACTGCTGTGCTG(+ACTCAC)GATCCAAAGGCCTCCTCACCAGGAAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240604191202_keud GGGCCAGAGCCTTCCTGGTGAGGAGGCCTTTG(+ACTCAC)GATCCAGCACAGCAGTTCCAGGAGGGCAGTG NGG 10 8 17 8 24 0 100 5 10