Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604191129_fzd5 CTTTGCCAGCCCCCTACCCCTTTATTATCTCG(+ACTCAC)GATCCCATCTTCTCCAGCAGCAGGCACCAAG NGG 10 8 17 8 24 0 100 5 10
    240604191128_w6sn CTCGCCTTGGTGCCTGCTGCTGGAGAAGATGG(+ACTCAC)GATCCGAGATAATAAAGGGGTAGGGGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240604191126_vhvj ACTGTTGTTGTCTTCAGACACACCAGAAGAGG(+ACTCAC)GATCCCATTATGGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240604191124_8ee7 ACCACATGGTGGCTCACAACCATCCATAATGG(+ACTCAC)GATCCCTCTTCTGGTGTGTCTGAAGACAACA NGG 10 8 17 8 24 0 100 5 10
    240604191123_5ruu TTTCTATGTAATCCAAGTGCCTCCTCCGGCTG(+ACTCAC)GTACCAGGATGGGAACAGAAGCCGGTTTATC NGG 10 8 17 8 24 0 100 5 10
    240604191121_w9f3 CACCTGATAAACCGGCTTCTGTTCCCATCCTG(+ACTCAC)GTACCAGCCGGAGGAGGCACTTGGATTACAT NGG 10 8 17 8 24 0 100 5 10
    240604191119_d9uj CTAGGCTAGGAGCTGGAGCTGTCTCCCTCCTG(+ACTCAC)GATCCAATAAAGCCAGATGCTTTGCCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240604191118_fhot TGGAGCTGGAGGCAAAGCATCTGGCTTTATTG(+ACTCAC)GATCCAGGAGGGAGACAGCTCCAGCTCCTAG NGG 10 8 17 8 24 0 100 5 10
    240604191115_53tv CTATCTGCACACTGACAACAAGAGGGCCCTGG(+ACTCAC)GATCCAGTGGGAAATGACGTGGGAATGAAGG NGG 10 8 17 8 24 0 100 5 10
    240604191113_jd22 AAAAACCTTCATTCCCACGTCATTTCCCACTG(+ACTCAC)GATCCCAGGGCCCTCTTGTTGTCAGTGTGCA NGG 10 8 17 8 24 0 100 5 10