Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604191112_175r ATCACAATCATCTGTAACTTAGGTTCCAGAGG(+ACTCAC)GATCCAATGCCCTCTTCTGACTTCTGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240604191110_t1mf TTACCGGCTGCAGAAGTCAGAAGAGGGCATTG(+ACTCAC)GATCCCTCTGGAACCTAAGTTACAGATGATT NGG 10 8 17 8 24 0 100 5 10
    240604191109_7y3r AATGTCTGAACCCTGGCTTTCAATTTTTTTAG(+ACTCAC)GTACCCTACTTAGAGATGGACTTGCTGGATT NGG 10 8 17 8 24 0 100 5 10
    240604191107_wb7s CATGTAATCCAGCAAGTCCATCTCTAAGTAGG(+ACTCAC)GTACCTAAAAAAATTGAAAGCCAGGGTTCAG NGG 10 8 17 8 24 0 100 5 10
    240604191106_n8w3 ATCTGACTGAGATTTGCCAAGTGGATACTGGG(+ACTCAC)GTACCACTGCCCCCCAAGAAAAGACTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240604191104_g7r1 TTCCTGGCTCAGTCTTTTCTTGGGGGGCAGTG(+ACTCAC)GTACCCCAGTATCCACTTGGCAAATCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240604191103_s1a1 CACAGGGCCCAGATGGTGGGTAACTGAGGCAG(+ACTCAC)GATCCAGACTTGAGACACACGGTTGGAAACA NGG 10 8 17 8 24 0 100 5 10
    240604191101_scxk AGCCTTGTTTCCAACCGTGTGTCTCAAGTCTG(+ACTCAC)GATCCTGCCTCAGTTACCCACCATCTGGGCC NGG 10 8 17 8 24 0 100 5 10
    240604191100_4ngc GCCTTGTCTAGGAACACAGAAAGGAGATGATG(+ACTCAC)GTACCTACTGGCCTTGGACTCTTCTGCGTGA NGG 10 8 17 8 24 0 100 5 10
    240604191059_nxu4 GTTTGTCACGCAGAAGAGTCCAAGGCCAGTAG(+ACTCAC)GTACCATCATCTCCTTTCTGTGTTCCTAGAC NGG 10 8 17 8 24 0 100 5 10