Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604190808_ghos ATTCAGTAACTTAAATATTGCCAGTGACTGAG(+ACTCAC)GATCCCCTCCAGTCATGGTTCTGTATATTCT NGG 10 8 17 8 24 0 100 5 10
    240604190806_n6td CTCAAAGAATATACAGAACCATGACTGGAGGG(+ACTCAC)GATCCTCAGTCACTGGCAATATTTAAGTTAC NGG 10 8 17 8 24 0 100 5 10
    240604190805_31ov ATAGATACTTTGCATTTATGGCTTGAATGAAG(+ACTCAC)GTACCAATTTTGATCTGGAGTTCTAAATGTG NGG 10 8 17 8 24 0 100 5 10
    240604190803_qcyy ATTTTCACATTTAGAACTCCAGATCAAAATTG(+ACTCAC)GTACCTTCATTCAAGCCATAAATGCAAAGTA NGG 10 8 17 8 24 0 100 5 10
    240604190802_iff5 GGCGGCTGTGGTCCAGCAGGATCTACATGTAG(+ACTCAC)GTACCTACTCTTTGGTGCAATAGTGTTACGA NGG 10 8 17 8 24 0 100 5 10
    240604190801_a0i9 ATCTTTCGTAACACTATTGCACCAAAGAGTAG(+ACTCAC)GTACCTACATGTAGATCCTGCTGGACCACAG NGG 10 8 17 8 24 0 100 5 10
    240604190759_l0db TAGTTGACCTAGCCTTTCTTGGTGGTTGGTTG(+ACTCAC)GTACCTTTGATGAGATGTTAGAGAATTCTGT NGG 10 8 17 8 24 0 100 5 10
    240604190758_56o3 GAATTACAGAATTCTCTAACATCTCATCAAAG(+ACTCAC)GTACCAACCAACCACCAAGAAAGGCTAGGTC NGG 10 8 17 8 24 0 100 5 10
    240604190757_wwu1 GTGCCCTGGTGCTGCAGGATTCCAGACTGTAG(+ACTCAC)GATCCACACAGTGAGCTAACACCCCCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240604190755_iumg CTTCATCTGAGGGGGTGTTAGCTCACTGTGTG(+ACTCAC)GATCCTACAGTCTGGAATCCTGCAGCACCAG NGG 10 8 17 8 24 0 100 5 10