Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604190736_b36y CACAAATGACTAAGCACCTGGCAGGGGCTGGG(+ACTCAC)GTACCTTTCAGCAGAGCCTTTGCTGAGCATG NGG 10 8 17 8 24 0 100 5 10
    240604190734_xa2j TGCTTCATGCTCAGCAAAGGCTCTGCTGAAAG(+ACTCAC)GTACCCCAGCCCCTGCCAGGTGCTTAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240604190732_a349 CCTGGAGCGCTCTCTCCTGGAGGCAGCGAGGG(+ACTCAC)GTACCACCTCTGCAAATAGACCAGGAAGCGG NGG 10 8 17 8 24 0 100 5 10
    240604190731_3xf9 TGGCACCGCTTCCTGGTCTATTTGCAGAGGTG(+ACTCAC)GTACCCCTCGCTGCCTCCAGGAGAGAGCGCT NGG 10 8 17 8 24 0 100 5 10
    240604190729_qll6 ATGGACTTCCTTGTCTAAAGGCCACCTCGAGG(+ACTCAC)GTACCACCTCAACATGCCAGGGTACTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240604190727_0qmp TGAAGAAAACAGTACCCTGGCATGTTGAGGTG(+ACTCAC)GTACCCTCGAGGTGGCCTTTAGACAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240604190726_76sw AGACTGCCAAGTCCGTTCATGTCGCAAGATGG(+ACTCAC)GATCCTGTGGCCCTGAGCCTAATAGTGCCAC NGG 10 8 17 8 24 0 100 5 10
    240604190724_4byb GGATGGTGGCACTATTAGGCTCAGGGCCACAG(+ACTCAC)GATCCCATCTTGCGACATGAACGGACTTGGC NGG 10 8 17 8 24 0 100 5 10
    240604190721_v2k0 GGTGTGTTTTTTTTTTTTTCTTTTTCCCCTTG(+ACTCAC)GTACCTATTGGCATTCTGTAAATCACACTAT NGG 10 8 17 8 24 0 100 5 10
    240604190719_sefd ACCACATAGTGTGATTTACAGAATGCCAATAG(+ACTCAC)GTACCAAGGGGAAAAAGAAAAAAAAAAAAAC NGG 10 8 17 8 24 0 100 5 10