Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604190642_6xo9 CATCACAGCAATAGTAACCCTAATGAAGACAG(+ACTCAC)GATCCCAGCACCCATGCTGGGTAGCTTACAA NGG 10 8 17 8 24 0 100 5 10
    240604190640_b0o0 TGCTGTTGTAAGCTACCCAGCATGGGTGCTGG(+ACTCAC)GATCCTGTCTTCATTAGGGTTACTATTGCTG NGG 10 8 17 8 24 0 100 5 10
    240604190638_hx27 TAAGAGAGTTTATTCGGGTTCTGCACTCAGAG(+ACTCAC)GTACCTACAACAGACTACAGACAAAATCAAT NGG 10 8 17 8 24 0 100 5 10
    240604190636_3x1j TCCTAATTGATTTTGTCTGTAGTCTGTTGTAG(+ACTCAC)GTACCTCTGAGTGCAGAACCCGAATAAACTC NGG 10 8 17 8 24 0 100 5 10
    240604190635_qe37 GACTTTGTACTCTGGCTGTATCCCATCAGAAG(+ACTCAC)GTACCATTGTAAGACTCTGGTGAGCCATAGC NGG 10 8 17 8 24 0 100 5 10
    240604190633_id4i GGTAAGCTATGGCTCACCAGAGTCTTACAATG(+ACTCAC)GTACCTTCTGATGGGATACAGCCAGAGTACA NGG 10 8 17 8 24 0 100 5 10
    240604190631_a5p6 CTCACCCCTCCCTTGGCATCCCTATAACTAGG(+ACTCAC)GTACCAAGCATGAAGTTCCAGCATGGGGAGT NGG 10 8 17 8 24 0 100 5 10
    240604190630_c24c GAATAACTCCCCATGCTGGAACTTCATGCTTG(+ACTCAC)GTACCCTAGTTATAGGGATGCCAAGGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240604190628_03d9 TCAGATTGTCACAGGAATACCCCACTCTGCTG(+ACTCAC)GTACCAATTTGTCTTAGTCAGGGTTTCTATT NGG 10 8 17 8 24 0 100 5 10
    240604190626_kjha GCAGGAATAGAAACCCTGACTAAGACAAATTG(+ACTCAC)GTACCAGCAGAGTGGGGTATTCCTGTGACAA NGG 10 8 17 8 24 0 100 5 10