Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604190527_oeqc ATCCTGCTGGTGATACCTAGGAGCTTCAAGGG(+ACTCAC)GTACCTAAGTGCTCCAAGAGGTACGTTACAG NGG 10 8 17 8 24 0 100 5 10
    240604190525_8hr3 GGACCCTGTAACGTACCTCTTGGAGCACTTAG(+ACTCAC)GTACCCCTTGAAGCTCCTAGGTATCACCAGC NGG 10 8 17 8 24 0 100 5 10
    240604190524_zxko AGGTGAGCGGGTCTGGACTGAGAACACGGCTG(+ACTCAC)GATCCCCATAACATGAGGGATTAGAACAAAT NGG 10 8 17 8 24 0 100 5 10
    240604190522_dxsr CAGGGATTTGTTCTAATCCCTCATGTTATGGG(+ACTCAC)GATCCAGCCGTGTTCTCAGTCCAGACCCGCT NGG 10 8 17 8 24 0 100 5 10
    240604190520_2cjr AGGTGGGTGGGAACCAGGTGGCGCAGCTTTGG(+ACTCAC)GTACCGCTGCCTTCTGGAACTGGTAAAGCAC NGG 10 8 17 8 24 0 100 5 10
    240604190517_7cvm CACTTGTGCTTTACCAGTTCCAGAAGGCAGCG(+ACTCAC)GTACCCAAAGCTGCGCCACCTGGTTCCCACC NGG 10 8 17 8 24 0 100 5 10
    240604190515_pb0h GCACACAGCCTGCACCCGTACTTCCATGTTGG(+ACTCAC)GATCCCGTCCCTGTGCTGAGTGACTTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240604190513_dtb9 CCAACCCTGCAAGTCACTCAGCACAGGGACGG(+ACTCAC)GATCCCAACATGGAAGTACGGGTGCAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240604190510_ys3c TCCTGGCACACAGTAGGTGGCTGTCCCCCGTG(+ACTCAC)GTACCACAGTACAGACGGAAGGCGTTCTTGA NGG 10 8 17 8 24 0 100 5 10
    240604190508_j9q5 GAGTGTCAAGAACGCCTTCCGTCTGTACTGTG(+ACTCAC)GTACCACGGGGGACAGCCACCTACTGTGTGC NGG 10 8 17 8 24 0 100 5 10