Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604190606_mk7d ATAGCAGGTCCTTAAGTGGGAATGAGCTCAGG(+ACTCAC)GATCCTCAGCCAGCCATGCAGGCAGGGCGCA NGG 10 8 17 8 24 0 100 5 10
    240604190604_937z GTCGTTGCGCCCTGCCTGCATGGCTGGCTGAG(+ACTCAC)GATCCCTGAGCTCATTCCCACTTAAGGACCT NGG 10 8 17 8 24 0 100 5 10
    240604190602_rbit ACAGCATAGCAGAAGTTTTTAATAGGTGACTG(+ACTCAC)GTACCACAGTAAGAAGGAATCATTTCGCCTG NGG 10 8 17 8 24 0 100 5 10
    240604190601_f9vf ACGGGCAGGCGAAATGATTCCTTCTTACTGTG(+ACTCAC)GTACCAGTCACCTATTAAAAACTTCTGCTAT NGG 10 8 17 8 24 0 100 5 10
    240604190559_kysl TCAGCTGTGCTCCAGATGATACAACGCAGTTG(+ACTCAC)GTACCTGCAGCCACTGTGCTCTGAGGCTAGG NGG 10 8 17 8 24 0 100 5 10
    240604190557_m1lw CTCACCCTAGCCTCAGAGCACAGTGGCTGCAG(+ACTCAC)GTACCAACTGCGTTGTATCATCTGGAGCACA NGG 10 8 17 8 24 0 100 5 10
    240604190554_forj TCTCATTGCTTCAGCCTCTTCTCTGCTGGGTG(+ACTCAC)GATCCTGTTTCTGACTGTGGCTGTGTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240604190553_7obo AAAGCAGAAGACACAGCCACAGTCAGAAACAG(+ACTCAC)GATCCACCCAGCAGAGAAGAGGCTGAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240604190551_8xte CATGAGCCGGGCTGGAAACTTCGTTGATGTGG(+ACTCAC)GATCCAGGCTCCATATGTAGTCCTGGAGGAT NGG 10 8 17 8 24 0 100 5 10
    240604190549_t0a9 CTGCTATCCTCCAGGACTACATATGGAGCCTG(+ACTCAC)GATCCCACATCAACGAAGTTTCCAGCCCGGC NGG 10 8 17 8 24 0 100 5 10