Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604070636_gew4bsnd GGAAGTGGACATGGCAGCGAGTCGAACAGCAGCTCTGAATCTTCTGAGAGG(/A)CAGTCGGAATCTGAGAGCGAATCAGCAGGTTCCAAATCCCAGCCAGTCC NGG 10 8 17 8 24 0 100 5 10
    240604070636_gew4am0j CAGCTCTGAATCTTCTGAGAGTCAGTCGGAATCTGAGAGCGAATCAGCAGG(/T)TTCCAAATCCCAGCCAGTCCTCCCAGAAGCCAAAGAGAAGCCAGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240604070636_gew4ae48 AATCTCTCTCTCTCTCAAAATAAGTCACTCAGCCTCTGAAGAAGCTTCGGG(/A)TTCAGACTCAGGCAGTCAGTCGGAAAGTGAGCAGGGAAGTGATCCAGGA NGG 10 8 17 8 24 0 100 5 10
    240604070636_gew49h1a TGATAAAGTGAAGACTTAGCCTTTTGTTTCTCTTCTCATTTTAGAACAGTT(/C)CCCAAACCTCGTGTTAAAAAGCAGCCGAAGACTCAGCGTGGAAAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240604070636_gew488ql TGGACATGGCAGCGAGTCGAACAGCAGCTCTGAATCTTCTGAGAGTCAGTT(/A)GGAATCTGAGAGCGAATCAGCAGGTTCCAAATCCCAGCCAGTCCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240604070636_gew47ure TGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCAGAAGACCTGTCCC(/T)CAGAAGGTGCACTGTTTGCTTAAGATCTCTACTTGGGATGAGCTTAATC NGG 10 8 17 8 24 0 100 5 10
    240604070636_gew47tdu ACCAGTGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCAGAAGACCC(/G)GTCCCCAGAAGGTGCACTGTTTGCTTAAGATCTCTACTTGGGATGAGCT NGG 10 8 17 8 24 0 100 5 10
    240604070636_gew47iql CACCAGTGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCAGAAGACC(/T)TGTCCCCAGAAGGTGCACTGTTTGCTTAAGATCTCTACTTGGGATGAGC NGG 10 8 17 8 24 0 100 5 10
    240604070636_gew45og3 ATCACAGAGAAAAATGGAAACAGGAACCCTCAGAAGATGAACAGGAACAAA(/C)GCACCAGTGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240604070636_gew45nmz CCGAAGAACTGTGAATTAAAGTCTGTAATCATTTTTCCCCCCTCACACACC(/A)GAAGGCAAGTAGCGGGTCTGAGAGTGGGAGCCCAAAAAGAAGAGGCCAG NGG 10 8 17 8 24 0 100 5 10