Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604070636_gew4x7w7 AGAAAAAAATTACTTTGTTTTGCTTTTGATCACAGAGAAAAATGGAAACAA(/G)GAACCCTCAGAAGATGAACAGGAACAAGGCACCAGTGCAGAGAGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240604070636_gew4wt60 ATTTTAGAACAGTGCCCAAACCTCGTGTTAAAAAGCAGCCGAAGACTCAGG(/C)GTGGAAAGAGAAAAAAGCAAGATTCTTCtgatgaggatgatgatgatga NGG 10 8 17 8 24 0 100 5 10
    240604070636_gew4vwle AAGGACAAAAGCCAAGAGGAGGACAGTTCGCTACACAGCAATGCATCGAGG(/C)TATGAGCTATCAACTTTCTTCCTTTTTGTCTTTTTTTTTGGGGGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240604070636_gew4vsk4 CAGATTCCCCCTCCCCCTTAATATTTAAGAATTAAAAGATGATGAGAAATT(/G)AGGACAAAAGCCAAGAGGAGGACAGTTCGCTACACAGCAATGCATCGAG NGG 10 8 17 8 24 0 100 5 10
    240604070636_gew4uqhj TCTGAATCTTCTGAGAGTCAGTCGGAATCTGAGAGCGAATCAGCAGGTTCC(/T)AAATCCCAGCCAGTCCTCCCAGAAGCCAAAGAGAAGCCAGCCTCTAAGA NGG 10 8 17 8 24 0 100 5 10
    240604070636_gew4toux GGACATGGCAGCGAGTCGAACAGCAGCTCTGAATCTTCTGAGAGTCAGTCC(/T)GAATCTGAGAGCGAATCAGCAGGTTCCAAATCCCAGCCAGTCCTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240604070636_gew4swxg ATATCCTGATGTTTATGGGGTCAGGCGGTCAAACCGAAGCAGACAAGAACC(/T)ATCGCGATTTAATATTAAGGAAGAGGTAAGGAAAAAATGTTTTAAGGGC NGG 10 8 17 8 24 0 100 5 10
    240604070636_gew4sr1w CAGTCGGAATCTGAGAGCGAATCAGCAGGTTCCAAATCCCAGCCAGTCCTT(/C)CCAGAAGCCAAAGAGAAGCCAGCCTCTAAGAAGGAACGGATAGCTGATG NGG 10 8 17 8 24 0 100 5 10
    240604070636_gew4sksj ATAAAGTGAAGACTTAGCCTTTTGTTTCTCTTCTCATTTTAGAACAGTGCC(/T)CAAACCTCGTGTTAAAAAGCAGCCGAAGACTCAGCGTGGAAAGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604070636_gew4ryj0 CAGTCAGTCGGAAAGTGAGCAGGGAAGTGATCCAGGAAGTGGACATGGCAA(/G)CGAGTCGAACAGCAGCTCTGAATCTTCTGAGAGTCAGTCGGAATCTGAG NGG 10 8 17 8 24 0 100 5 10