Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604070433_6fe56cv5 ACAAGGCACCAGTGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCAA(/G)AAGACCTGTCCCCAGAAGGTGCACTGTTTGCTTAAGATCTCTACTTGGG NGG 10 8 17 8 24 0 100 5 10
    240604070433_6fe569ku CAAAATAAGTCACTCAGCCTCTGAAGAAGCTTCGGGTTCAGACTCAGGCAA(/G)TCAGTCGGAAAGTGAGCAGGGAAGTGATCCAGGAAGTGGACATGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240604070433_6fe55uf6 CTTAATATTTAAGAATTAAAAGATGATGAGAAATAAGGACAAAAGCCAAGG(/A)GGAGGACAGTTCGCTACACAGCAATGCATCGAGGTATGAGCTATCAACT NGG 10 8 17 8 24 0 100 5 10
    240604070433_6fe55cr7 TTTGATCACAGAGAAAAATGGAAACAGGAACCCTCAGAAGATGAACAGGAA(/G)CAAGGCACCAGTGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCA NGG 10 8 17 8 24 0 100 5 10
    240604070433_6fe54nen AAGGCAAGTAGCGGGTCTGAGAGTGGGAGCCCAAAAAGAAGAGGCCAGAGG(/A)CAGCTGAAAAAACAGTAAGTCTTTCATGGGGGAAATTATTCAATCTAGT NGG 10 8 17 8 24 0 100 5 10
    240604070433_6fe54cta CCCTCCCCCTTAATATTTAAGAATTAAAAGATGATGAGAAATAAGGACAAA(/G)AGCCAAGAGGAGGACAGTTCGCTACACAGCAATGCATCGAGGTATGAGC NGG 10 8 17 8 24 0 100 5 10
    240604070433_6fe546mm AATCTCTCTCTCTCTCAAAATAAGTCACTCAGCCTCTGAAGAAGCTTCGGG(/A)TTCAGACTCAGGCAGTCAGTCGGAAAGTGAGCAGGGAAGTGATCCAGGA NGG 10 8 17 8 24 0 100 5 10
    240604070433_6fe540oe GAGCGAATCAGCAGGTTCCAAATCCCAGCCAGTCCTCCCAGAAGCCAAAGG(/A)GAAGCCAGCCTCTAAGAAGGAACGGATAGCTGATGTGAAGAAGGTATCT NGG 10 8 17 8 24 0 100 5 10
    240604070433_6fe52r7s CAGTCAGTCGGAAAGTGAGCAGGGAAGTGATCCAGGAAGTGGACATGGCAA(/G)CGAGTCGAACAGCAGCTCTGAATCTTCTGAGAGTCAGTCGGAATCTGAG NGG 10 8 17 8 24 0 100 5 10
    240604070433_6fe51prb TTCCAAATCCCAGCCAGTCCTCCCAGAAGCCAAAGAGAAGCCAGCCTCTAA(/G)GAAGGAACGGATAGCTGATGTGAAGAAGGTATCTACTTTGCCCTGCAGT NGG 10 8 17 8 24 0 100 5 10