Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604070433_6fe51jrv CTCTGAATCTTCTGAGAGTCAGTCGGAATCTGAGAGCGAATCAGCAGGTTT(/G)CAAATCCCAGCCAGTCCTCCCAGAAGCCAAAGAGAAGCCAGCCTCTAAG NGG 10 8 17 8 24 0 100 5 10
    240604070433_6fe51c43 TCTCTCAAAATAAGTCACTCAGCCTCTGAAGAAGCTTCGGGTTCAGACTCC(/T)GGCAGTCAGTCGGAAAGTGAGCAGGGAAGTGATCCAGGAAGTGGACATG NGG 10 8 17 8 24 0 100 5 10
    240604070433_6fe51aiu GCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCAGAAGACCTGTCCCC(/T)AGAAGGTGCACTGTTTGCTTAAGATCTCTACTTGGGATGAGCTTAATCT NGG 10 8 17 8 24 0 100 5 10
    240604070433_6fe515g5 GAAGGATTCTTTTAGAAAAAAATTACTTTGTTTTGCTTTTGATCACAGAGG(/C)AAAATGGAAACAGGAACCCTCAGAAGATGAACAGGAACAAGGCACCAGT NGG 10 8 17 8 24 0 100 5 10
    240604070433_6fe50654 CAGTCGGAATCTGAGAGCGAATCAGCAGGTTCCAAATCCCAGCCAGTCCTT(/C)CCAGAAGCCAAAGAGAAGCCAGCCTCTAAGAAGGAACGGATAGCTGATG NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywzzrpl AGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCAGAAGACCTGTCCCCAA(/G)AAGGTGCACTGTTTGCTTAAGATCTCTACTTGGGATGAGCTTAATCTGC NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywzyyjf TGTAATATACTCTCTTTCAAGTGTACTTAATCCTTTTGCAGCCACTGGAGG(/A)ATCTACTACTGTATATGCGATTGAAGCTAATGGCGACCCTAGTGGTGAC NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywzyxaw CCCTCCCCCTTAATATTTAAGAATTAAAAGATGATGAGAAATAAGGACAAA(/G)AGCCAAGAGGAGGACAGTTCGCTACACAGCAATGCATCGAGGTATGAGC NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywzyw2g TAAATCTCTCTCTCTCTCAAAATAAGTCACTCAGCCTCTGAAGAAGCTTCC(/T)GGTTCAGACTCAGGCAGTCAGTCGGAAAGTGAGCAGGGAAGTGATCCAG NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywzypw2 CCAAAAGGCAGACTCGTCGAAGAGCGGCTAAAAACGTTAGGTAAGTTGTAA(/G)CCCAGAAGTGTTTCACTGGTGTGATGTGATAAAGTAGTTGGTTAGATTT NGG 10 8 17 8 24 0 100 5 10