Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604070312_cywzgsy2 AGAACAGTGCCCAAACCTCGTGTTAAAAAGCAGCCGAAGACTCAGCGTGGG(/A)AAGAGAAAAAAGCAAGATTCTTCtgatgaggatgatgatgatgacgaAG NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywzgq6s AAAAAAGCAAGATTCTTCtgatgaggatgatgatgatgacgaAGCTCCCAA(/C)AAGGCAGACTCGTCGAAGAGCGGCTAAAAACGTTAGGTAAGTTGTACCC NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywzgm22 gacgaAGCTCCCAAAAGGCAGACTCGTCGAAGAGCGGCTAAAAACGTTAGG(/A)TAAGTTGTACCCCAGAAGTGTTTCACTGGTGTGATGTGATAAAGTAGTT NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywzghmj CTCTGAATCTTCTGAGAGTCAGTCGGAATCTGAGAGCGAATCAGCAGGTTT(/G)CAAATCCCAGCCAGTCCTCCCAGAAGCCAAAGAGAAGCCAGCCTCTAAG NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywzg53i TCAGCATTATTCCTCTTGCAGTTACAAAGAAGATGATGACTTTGAGACTGG(/C)CTCAGATGATCTCATTGAAATGACTGGAGAAGGAGTTGATGAACAGCAA NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywzfj1o ACAGATTCCCCCTCCCCCTTAATATTTAAGAATTAAAAGATGATGAGAAAA(/G)AAGGACAAAAGCCAAGAGGAGGACAGTTCGCTACACAGCAATGCATCGA NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywzf6dw TGACTTTGAGACTGACTCAGATGATCTCATTGAAATGACTGGAGAAGGAGG(/A)TGATGAACAGCAAGATAATAGTGAAACTATTGAAAAGGTCTTAGATTCA NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywzf1m9 CCTGATGTTTATGGGGTCAGGCGGTCAAACCGAAGCAGACAAGAACCATCC(/T)CGATTTAATATTAAGGAAGAGGTAAGGAAAAAATGTTTTAAGGGCATGC NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywzf0p5 ATTTTAGAACAGTGCCCAAACCTCGTGTTAAAAAGCAGCCGAAGACTCAGG(/C)GTGGAAAGAGAAAAAAGCAAGATTCTTCtgatgaggatgatgatgatga NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywzeywm TAATAGTGAAACTATTGAAAAGGTCTTAGATTCAAGACTGGGAAAGAAAGG(/A)AGGTATGTGTATTTGGGGAGCAAATTACATTTTATGTATGCTTAGTAAG NGG 10 8 17 8 24 0 100 5 10