Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604070312_cywz6jl6 ATATCCTGATGTTTATGGGGTCAGGCGGTCAAACCGAAGCAGACAAGAACC(/T)ATCGCGATTTAATATTAAGGAAGAGGTAAGGAAAAAATGTTTTAAGGGC NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywz69gy CACTGAAAAGGATGAAGGTGAAATCCAGTACCTCATCAAGTGGAAGGGTTT(/C)GTCTTACATCCACAGCACATGGGAGAGTGAAGAATCCTTACAGCAACAG NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywz646a TAGAAAAAAATTACTTTGTTTTGCTTTTGATCACAGAGAAAAATGGAAACC(/G)GGAACCCTCAGAAGATGAACAGGAACAAGGCACCAGTGCAGAGAGTGAG NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywz5rhd AAAAATGGAAACAGGAACCCTCAGAAGATGAACAGGAACAAGGCACCAGTT(/G)CAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCAGAAGACCTGTCCC NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywz57o1 gatgaggatgatgatgatgacgaAGCTCCCAAAAGGCAGACTCGTCGAAGG(/A)GCGGCTAAAAACGTTAGGTAAGTTGTACCCCAGAAGTGTTTCACTGGTG NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywz4rm9 TTCCAAATCCCAGCCAGTCCTCCCAGAAGCCAAAGAGAAGCCAGCCTCTAA(/G)GAAGGAACGGATAGCTGATGTGAAGAAGGTATCTACTTTGCCCTGCAGT NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywz4iey CAGATTCCCCCTCCCCCTTAATATTTAAGAATTAAAAGATGATGAGAAATT(/G)AGGACAAAAGCCAAGAGGAGGACAGTTCGCTACACAGCAATGCATCGAG NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywz4c1h AGATCATTTCTCATTTATTCAGCATTATTCCTCTTGCAGTTACAAAGAAGG(/C)TGATGACTTTGAGACTGACTCAGATGATCTCATTGAAATGACTGGAGAA NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywz407r ATGAACAGCAAGATAATAGTGAAACTATTGAAAAGGTCTTAGATTCAAGAA(/C)TGGGAAAGAAAGGAGGTATGTGTATTTGGGGAGCAAATTACATTTTATG NGG 10 8 17 8 24 0 100 5 10
    240604070312_cywz3r7j GATCTCATTGAAATGACTGGAGAAGGAGTTGATGAACAGCAAGATAATAGG(/A)GAAACTATTGAAAAGGTCTTAGATTCAAGACTGGGAAAGAAAGGAGGTA NGG 10 8 17 8 24 0 100 5 10