Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604070312_cywz0i0k CAAAATAAGTCACTCAGCCTCTGAAGAAGCTTCGGGTTCAGACTCAGGCAA(/G)TCAGTCGGAAAGTGAGCAGGGAAGTGATCCAGGAAGTGGACATGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240604064743_4bmjxnas CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240604064743_4bmjoqjw CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240604064743_4bmjc72w CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240603203149_3mob GCCCACTGAAGGGCATCTTGGGCTACACTGAGGACCAGGTTGTCTCCTGCGACTTCAACAGCAACTCCCACTCTTCCACCTTCGATGCCGGGGCTGGCATTGCTCTCAATGACAACTTTGTCAAGCTCATTTCCTGGTATGTGGGGATGGGAAACCTGACTTTTAAGAGCAACTGGGGGTTTGGTGCCCTCTGGTGGCTAGCTCAGAAAAGAAACCCAAACTAACAGTTGTCCCAATTTGTTCTAGGTATGACAATGAATACGGCTACAGCAACAGGGTGGTGGACCTCATGGCCTACATGGCCTCCAAGGAG(+ggaagc)TAAGAAACCCTGGACCACCCACCCCAGCAAGGACACTGAGCAAGAGAGGCCCTATCCCAACTCGGCCCCCAACACTGAGCATCTCCCTCACAATTTCCATCCCAGACCCCCATAATAACAGGAGGGGCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTGCACCCACCTCTCCTTGGTTTTAATGCGGTGTTGGGGGAGGGAGGGTGTGTATGGGGAGGTGAAGCAGGCTCAATCAACTTTTGTGCATGTATCTTTATTGGCTCTGACATCCAGCTGGGTGCCGGAAGTCCAGGGCTACATTCTATCCAAGTCCCAGCCCAGGGCGCCTGTCCTGAACAGCTGATATGTCTGGCAATGCGGAGGGTGGGGCTTGACAATGGGACAGGAAATGCAGTCTGAGACTCACTTGTTGGACAGCACTGACTTCCAGAGCTCACTGCTCTTGAGAAAGATCGGTGTCCTTTTATCTG NGG 10 8 17 8 24 0 100 5 10
    240603185541_27o7 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240603185509_7tos CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240603185434_cnrh CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240603154814_devt ACGTCGCGCAGAAGCTAGAGCAGCTCGAGATGGCCATGGGGATGGGCGGCGCCTGCCCCACCGCTGATGACGGGTTCGTCTCGCACCTCGCCACGGACACCGTGCACTACAATCC(/A)CTCCGACCTGTCGTCCTGGGTCGAGAGCATGCTGTCCGAGCTCAACGCGCCCCCGCCGCCGCTCCCACCCGCGACGCCGGCACCAAGGCTGGCGTCCACCTCGTCCACCGTCACAAGTGGCGCCGCCGCCGGTGCCGGCTACTTCGATCTCCCGCCCGCCGTCGACTCGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240603154539_dz7d ATGAAGCGCGAGTACCAAAACGCCGGCGGGAACGACGGCTACAGGGGCTCCTCCAAGGACAAGTCGATGGCGGCGGCGGCGGGGGCAGGGGAGCAGGAGGAGGAGGTGGACGAGCTGCTGGCGGCGCTCGGGTACAAGG(/A)TGCGTTCGTCGGATATGGCGGACGTCGCGCAGAAGCTAGAGCAGCTCGAGATGGCCATGGGGATGGGCGGCGCCTGCCCCACCGCTGATGACGGGTTCGTCTCGCACCTCGCCACGGACACCGTGCACTACAATCCCTCCGACCTGTCGTCCTGGGTCGAGAGCATGCTGT NGG 10 8 17 8 24 0 100 5 10