Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240529181145_hbxe CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240529133958_zoh5 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240529133709_13mg CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240528192623_xxau CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240528191305_j71n CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240528183401_ylp6 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240528043237_814k GCCAGCTTTACCTCTCCACAGGCACCACTAGAGGGAAAAACAAAGATAGTGATTGTCTGAATGTTTTTAATTATGAGACCTACCACTCCTGGGTCCACTTCAAGTATTAGAATTTACAGACATTCCATATAACAGCCTTTCCATTGTCCCTGTTTGCAGAGGACACAGTGAAACTTCACCAGTGTAAAATGTCAAGGGCTTAACAGCCAAAAACACTAAGTCAGCTCCAAATTCCATTTCTGAGCCCAGCA(/g)ATACAAACATGGGTCTTACTGTAGCCCTGGATTGTGAAATTCCCAGAAAGATACTAAGAGAAAAATGAGAGAGAAACTACGTATCCGAGAGGTCAGAAAAACATTAACTAGAAACAGTCCTCCCTGGTCAAAAATAAGTGCCCAAAACAAACAAATAACGGTAAACAGAATCTGCAAATGAATAACTTTAATGGATAAATTTCTTTCCTTCTTATTCTCCATCTTCAAAGGAAGTGCATAAGCAGTTGAT NGG 10 8 17 8 24 0 100 5 10
    240528043043_l4jg GACAGCCAGGGCTGCCGCCTCCCGATCAGGCAGTGCACAGCCCTGGGACCCCCAGGGACACAGACCTAAGGGAAAGTGAGCCCCCAAACCTGCCTTCCAGCCAGCCTGCTTGGAGGAAATGCCCTAGCTTTATTGAACACTTAATTACTTAATAAATATTAGATGATACCATTCTCCACAAAAAAAAAAAAAAAAAAAAAAGATTGAAACCCAGAGAGAGATGGGCAAGTCACGAAAACAAGAATAAAGGG(/a)GATTATTCTTCAAGGTAACAGGATATGAAGAGAAGTGCCCCCAGGCTGGTCCGCTGGAGCATTTAGGACAGCAGAAGAGAGGCCTCTTTATAATCTCCCGACCTGAAATTCACCCTCATCGCTCCCAGCTTCACATACAACCTGGAACTCCGGGGGAGATGGGGAAAGCCGGGGGGCGGCCCCTGTGACCTGGCTGACCCAGGTCTCATGACAGAATGTGAAAGACTCCTTTATTTGTAATTCTCAGTAC NGG 10 8 17 8 24 0 100 5 10
    240528042911_rfsw CCAGGTTCAAGCGATTCCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCACCATGTCTGGCTAATTTTGTATTTTTTTTCAGTAGAGACGCGGTTTCTCCACGTTGGTCAGGCTTGTCCTGAACTCCTGACCTCAGGTGATCCGCCCTCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACTGGGCCCGGCCGGCTTTTTTTTTTTTTTTTTTTAAACAAGTTTTACCATGG(/a)AAGGCAGAAAGCCTTTGGGAAGGACATAGGAGTTGGCCAACCTTCTTAGGTAAATAGGGTTAAATAGAAGGAAAAATAGGGGGAGGCTTCGAAATCCTGTAACATAGCTAGAGAGTGGCAGGGCTGGAATTGAAACAGTTGTGTCTAACCCAAACCTTGTGCTTGTTGCTGTATACATATATACGTGTGTGTATATACGTATGTGTATATATGTGTGTGTGTGTATATGTATATACATATATACACACAC NGG 10 8 17 8 24 0 100 5 10
    240528042719_a0hj TACTGTCCTTCAATAGATCTAGATTAGAAGAGGAAACACAGACCATCCATGAAGTTCTTTAAAGATTTGAAGCCCCTTGAAATTGCTGCTGCTACGGGGCTGCCATGATGCTTCAATACAAAGTCCCTATCTCTTAGAAAGCCAGCTGTCTGTGTTTGGTTACCTGTGTGCATTTTCGGGGTGGGCACTCTTAGTCTAAAAGAAAAATATGTTTGCACTTTCTTTATGGGAGTGATTCAGAGACCATTGCG(/a)TAAGTCATCATTGAAGGCACATGGTGTGTTCATTGCGAGCCATGGGAAGACTCCTTGGGTACTTGTCAATGACTATTGTGCTCCTCCCTAGGGCCAAGGTGCAAATTCCTATTTTGTTTACTCTTTAAATAGAGTACAAGAGAAGGGAGAGTGAGGCCCCGCAAAACAGGACAAAGAGCGGGGCCGTGACTCTAAAAGATCTCAGAAGTATTTGCGTAGCTGCAACATGCATCTCATTTCAGGGAATCCC NGG 50 8 17 8 50 0 200 5 10