Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240527134324_gnoo GACTAATTTTTTTTTTTTTTTTGTATTTTTTAGTAGAGACAGGGTTTCACCGCGTTAGCCAGGATGGTCTTGATCTCCTGACCTCATGATCCACCCGCCTCTGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCCGGCCGCAAATCTCTTTTCTTGTTAAATTTCAAAGACACATGCACAGCAAATCTCTTTAAATGTCTGGCTTAATACAAGACCACTGGGTTTTCCCATCTACTTTTGCATTCAATTTTTTGCAATACATTGTTCTGGTTGAAGGATATGAAGAAATCTGGCCTCACACAGATACAGTATATGCAGTTGGA(/G)AGTTTCAAATCTTAAGTTTTTTTATGTTTGTTTGTTTGTTTTTGAGACAGGGTCTCACTCTGTCACCCAGGCTAGAGTGCAGTGGCATGAACATGGCTCACTGCAGTCCTGACCTTCCGGGCTCAAGAGATCCTCTCACCTCAGCCTCCTGAGCAGCTGGGACCACAGGCACACACCACCATTCCTGGCTAATGTTTTAGTTTTTCGTAGAGACACGGTCTCGCTGTGTTGACCAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240527133134_5zvr GATGTGACAACAAATAATCTGTCACTGTGTGTGGGTGTCAGAGGAGGAAGGGACAGACTTACAAGCTCTTTCTCTGGGCCGTACTGGCTTGGCCTCTCCATCCAGAGAGGTAGCCTGGGAAAGCAGCCAAGCACCCTGGCTTAGGGATGTTTGCGGTGGCCCCTTGGCTCTTCAGGGTGCCTTCTGCCATGC(/A)GCAGTGACGGCAGACTGAGTCACTGGCAGGTCTTCCCTGTCATACTGGCCTGCTCTGGCTCATTTGGACTAGAAGAGGAGATTGCAACAAGGGAGGTCCTGGCATGGGCATGTGACTAAGAGTCCCTTCAGCTCCAACCATCAGTTGTTGCTTTGTGCAGTGACTCAGTCCTGGAATAAGGCCTGCCATGGGGTCCCATGTGGAGTTCAAGTTCTTACCACCCATGATAGCCCAAATCAGGCAAGGAGAAAGCAGCAAGCAACCGCAGAACAACTGGTTGATTCTAAAAGCCACCATTCTGTCCACTGTGTGACC NGG 10 8 17 8 24 0 100 5 10
    240524235108_k2j7 gcggccgccatgttgcaaccgggaaggaaatgaatgggcagccgttaggaaagcctgccggtgactaaccctgc(+ATATGGGGAAGTGAATcaG)gctcctgcctcgatgggtggagtcgcgtgtggcggggaagtcaggtggagcgaggctagc NGG 10 8 17 8 24 0 100 5 10
    240524234943_1vt3 gcggccgccatgttgcaaccgggaaggaaatgaatgggcagccgttaggaaagcctgccggtgactaaccctgc(+ATATGGGGAAGTGAATcaG)gctcctgcctcgatgggtggagtcgcgtgtggcggggaagtcaggtggagcgaggctagc NGG 10 8 17 8 24 0 100 5 10
    240524000109_x3md gctagcctcgctccacctgacttccccgccacacgcgactccacccatcgaggcaggagc(+ATATGGGGAAGTGAATca)gcagggttagtcaccggcaggctttcctaacggctgcccattcatttccttcccggttgcaacatggcggccgc NGG 10 8 17 8 24 0 100 5 10
    240524000044_9qjz gctagcctcgctccacctgacttccccgccacacgcgactccacccatcgaggcaggagc(+ATATGGGGAtagac)gcagggttagtcaccggcaggctttcctaacggctgcccattcatttccttcccggttgcaacatggcggccgc NGG 10 8 17 8 24 0 100 5 10
    240524000029_jww3 gctagcctcgctccacctgacttccccgccacacgcgactccacccatcgaggcaggagc(+ATATGGGGA)gcagggttagtcaccggcaggctttcctaacggctgcccattcatttccttcccggttgcaacatggcggccgc NGG 10 8 17 8 24 0 100 5 10
    240523235855_5psi ggccagctagcctcgctccacctgacttccccgccacacgcgactccacccatcgaggcaggagc(+ATT)gcagggttagtcaccggcaggctttcctaacggctgcccattcatttccttcccggttgcaacatggcggccgc NGG 10 8 17 8 24 0 100 5 10
    240523143404_wq19 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240521131824_stqg GAAGCAGGTCTTTTTCAGTAGGGATTTCCTGTGACCAGACAAGTTCATCTGAGAGCCAGTTCTCACCACTGGAATTCTCAGGAATGGACCATGAGGACATCAGTGAGTCAGTGGAT(/C)GCAGCATACAACCTCCAGGACAGTTGCCTTACAGACTGTGATGTGGAAGATGGGACTATGGATGGCAATGATGAGGGGCACTCCTTTGAACTTTGTCCTTCTGAAGCTTCTCCTTATGTAAGGTCAAGGGAG NGG 10 8 17 8 24 0 100 5 10