Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240528034926_wx1r TGGCATGGTCAGTCTCACTTACTATATTGTCTCTTTTCAGGCAATAATTTACTGTAACTACATTAGTTCAAAAAATATTTAGCCAGAAAAACACAATAAGCGATTCATTTTGTCCTTCAATCTTCTGCTAAAGAGCACAGATTCTCCAAATCACCAGGCAATATGGAAAAGGCAAGGTCTGTGTACATAGAGTGGCCCATAAAAAATAGAATTTTGAACAGTGGTATCCTGGTAAATATTTAACATTTGCT(/c)TCTCTGAAAAAGTATATCTAAATTTGCTGATATAAAATATACGTAGCATACAATTTACAAGTAACTAATAGTAATATACAAAACATTTTGCTGCAAATTCCAAATAGGCAATTGCTTCTTATTGAATGCTTTTGCTGGTTTTTGCCAAACTCCTGTATCCCTAGACAAAATATGGATTCAATTGACAGACAAATATAGTTCCAACAAGAATGTTAGTTGATACTTTTGTTTATCTTAACAAGAAATATCA NG 10 8 17 8 24 0 100 5 10
    240528034616_52q6 AAGTCAAATTTAACATTTTGCTTTTTTCTATTATATATAATAAACTTATTTGCTCACTTCTAACATTTGTCATTTTCAGCCTTCATTTTACCAGCACAGACTCCAGCTCTCGGTATTTGGGCCTTAAGATCTTCACAAACAAACAAATCTTTCTGAGATAGAAAAAAAAAAAAACAGACCCAAAAATCTAACACGATAGTGACCTCCTACTAGGCTTTAAGTTTAAACGGATTAGGGCAGGAAGTTTTACA(/g)TTCCTGTCGTATTTGCTGAAGGTGTATGGCAGGGTGCGGCGCCTGTTGCAGGATTCCAGCAGGGCCTGCCCTGGCTCCCCAGGGCTTTGCAAGGAAGCTGGAGAAGTCCGCCACCTCGCTGTCACTGCACATCAGTAGACCCCTATCCCCACCCGCAAACTCAGGGCACCCACAGTCTCTCCAAGGACTGCAGTTTTGTCGCCAGATTGTGGGAACATCTGTCTTGAATCTAAAGAGACAAAACAGATGG NGG 10 8 17 8 24 0 100 5 10
    240528034323_8py5 ACTTTGAGAGGCTGAGGTGGGGAGATCACTTGAGGTCAGGAGTTTGAGACCAACCTGGGAATACAAAAATTAGCCAGCCGTAGTGGTTGGGTGCCTGTAATCCTAGCTACTCGGGAGGCTGAGGCAGGAGAATCTTTTGAACCTGGGAGGTGGAGGCTGCAGTGAGCTGAGATCGGGCCACTGCACTCCAGCCTAGGTGACAGAGCGAGACTCTGTCTCAAAACACAAAACAAAACAATTTAGGTCTTGGA(/c)AAGCTTAAATGGAACACAGATGCAATCCCAGTAATTTGTGAAGCAGAGTTGGAGGGACTGCTTGAGCCCAGGAGTTCGAGACCTCGAGACCAACCTGGGCAACACAGGGAGACCCCTAGCTCTACAAAAAAAAAAAAAAATTAGCCAGGCATGGTGGTGCACACCTATAGTACCAGCTACTTGGGAGGCTGAGGCGGGAGGACTGCTTGAACTCGGGAGGTCAGGCTGGATGAGCCTCAATCATGCCACT NGG 10 8 17 8 24 0 100 5 10
    240528021003_2kpm GAGCTTAGCTACAAGGGGGGCAACACTCGCACAGGGGCTGCAATTCTCCATGTGGCTGACCATGTCTTCCTGCCCCAGCTGGCCCGACCTGGTGTCCCCAG(/A)GGTGATCCCTACCCCTACCATGCCTCCCAAGATGACCCCAAATGAAGTGTCCAGGGGAACCGTGATTTGACCCCTGCACCTGTCCCAGGTCTGCATCCTG NGG 10 8 17 8 24 0 100 5 10
    240527212058_9o5e ATGCAGGTTCAGTGCCAGCAGAGCCCAGTGCTGGCAGGCAGCGCCACTTTGGTCGCCCTTGGGGCACTGGCCTTGTACGTCGCGAAGCCCTCCGGCTACGGGAAGCACACGGAGAGCCTGAAGCCGGCGGCTACCCGCCTGCCAGCCCGCGCCGCCTGGTTCCTGCAGGAGCTGCCTTCCTTCGCGGTGCCCGCGGGGATCCTCGCCCGGCAGCCCCTCTCCCTCTTCGGGCCACCTGGGACGGTACTTCTGGCCTCTTCTGCG(/C)TACATTACTTCCACAGGACATTTGTGTACTCACTGCTCAATCGAGGGAGGCCTTATCCAGCTATACTCATTCTCAGAGGCACTGCCTTCTGCACTGGAAATGGAGTCCTTCAAGGCTACTATCTGATTTACTGTGCTGAATACCCTGATGGGTGGTACACAGACATACGGTTTAGCTTGGGTGTCTTCTTATTTATTTTGGGAATGGGAATAAACATTCATAGTGACTATATATTGCGCCAGCTCAGGAAGCCTGGAGAAATCAGCTACAGGATTCCACAAGGTGGCTTGTTTACGTATGTTTCTGGAGCCAATTTCCTCGGTGAGATCATTGAATGGATCGGCTATGCCCTGGCCACTTGGTCCCTCCCAGCACTTGCATTTGCATTTTTCTCACTTTGTTTCCTTGGGCTGCGAGCTTTTCACCACCATAGGTTCTACCTCAAGATGTTTGAGGACTACCCCAAATCTCGGAAAGCCCTTATTCCATTCATCTTTTAAAGGAACCAAATTAAAAAGGAGCAGAGCTCCCACAATGCTGATGAAAACTGTCAAGCTGCTGAAACTGTAATTTTCATGATATAATAGTCCCGTATATATGTAATAGTAGGTCTCCTGGCGTTCTGCCAGCTGGCCTGGGGATTCTGAGTGGTGTCTGCTTAGAGTTTACTCCTACCCTTCCAGGGACCCCTATCCTGATCCCCAACTGAAGCTTCAAAAAGCCACTTTTCCAAATGGCGACAGTTGCTTCTTAGCTATTGCTCTGAGAAAGTACAAACTTCTCCTATGTCTTTCACCGGGCAATCCAAGTACATGTGGCTTCATACCCACTCCCTGTCAATGCAGGACAACTCTGTAATCAAGAATTTTTTGACTTGAAGGCAGTACTTATAGACCTTATTAAAGGTATGCATTTTATACATGTAACAGAGTAGCAGAAATTTAAACTCTGAAGCCACAAAGACCCAGAGCAAACCCACTCCCAAATGAAAACCCCAGTCATGGCTTCCTTTTTCTTGGTTAATTAG NGG 20 8 17 8 24 0 100 5 10
    240527155058_nuzd CAAAAAACTAAAAAACTAGACATTGGTTTAATGGAAACATGAGAGGCAAAGGGATTGTCTCTACAGTTCTACTGTATGAAATAAATAATAGCTTCTTTTGTTTGTTTGTGTTGTTTTTGTTTTTTTGAGACAGAGTCTCACTCTGTTGCCCAGGCTGGAGAGCAATGGCATGATCTTGGCTCACTGGAACCTCTGCCTCCTGGGTTCAAGCAGTTCTCCTGCCTCAGCCTCCAAGTAGCTAGGATTACAGGTGCTCTCCACCACACCTGGCTAATTTTTTGTATTTTCAGTAGAGACAAGGTTTTGCCATGTTGGCCAGGCTGGTCTGAAACT(/C)CCTGAGCCCTAGTGATCCATCCACCTTGGCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCCACTGCACCCGGCCAATAGTTTGGTTTATTAGATGCATCAATAATCAGGGCATTTTGTAGAGGAACCTACTTATCTTTTAATGGAGATAGCATGCAATGGCTACTTCATCTAATTCATTAAAATACTTTTTCTTCCTACATTTATTTATTTATGTATTTATTTTATTTTATTTTTATTTTGAGATGGAGTTTCACTCTTGTTGCCCAGGCTGGAGTGCATTGGTGTGATCTCAGCTCACCACGACCTCTGCCTCCCTGGTTCAAGTGATTCTCCTGCCTCAGCCT NGG 10 8 17 8 24 0 100 5 10
    240527145213_lzvl agtttgagagccggctggcggatgcgctgcaggaactgT(/C)gggcccagcatgaggaccaggtggagcagtataagaaggagctggagaagacttattctgc NGG 10 8 17 8 24 0 100 5 10
    240527145047_o4mg agtttgagagccggctggcggatgcgctgcaggaactgT(/C)gggcccagcatgaggaccaggtggagcagtataagaaggagctggagaagacttattctgc NGG 10 8 17 8 24 0 100 5 10
    240527140505_rqbs agtttgagagccggctggcggatgcgctgcaggaactgT(/C)gggcccagcatgaggaccaggtggagcagtataagaaggagctggagaagacttattctgc NGG 10 8 17 8 24 0 100 5 10
    240527140451_47l4 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10