Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240329105431_pndt aggtccctgctggtgccagcggccccccacagcgcgagtggcaggagcggttggtggtggggcacaacgtttgcttcga(/c)ccgagcgcatgtcagggagcagtacctgatccaggtatggg NGG 10 8 17 8 24 0 100 5 10
    240329100901_86pr accacatgagactctggactgtgaaagacttacggattccttctgcagatgatgtgcatgtcagctcattcataacttagtgtgtctttaaacaattcttagtgaaagtagtctgcttttaattgcttagattcatctttttccttttgtatgattgcaggtcctacag(/t)gtatggatctctggcagcttctgttgaccttggcagtggcaggctcaagtgatgctttttctgggagtgaaggtgagttctgcttttccattcccaccttcagtgtttttgaaaccacactgaa NGG 10 8 17 8 24 0 100 5 10
    240329012341_ro8k CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240328235906_nrqs CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240328171338_m6h6 GATCATCGTGGAGAACCTCTTCTACCCTGTGACCCTGGATGTGCTGCACCAGATTTTCTCCAAGTTCGGCACAGTGTTGAAGATCATCACCTTCACCAAGAACAACCAGTTCCAGGCCCTGCTGCAGTATGCGGACCCCGTGAGCGCCCAGCACGCCAAGCTGTCGCTGGACGG(/C)GCAGAACATCTACAACGCCTGCTGCACGCTGCGCATCGACTTTTCCAAGCTCACCAGCCTCAACGTCAAGTACAACAATGACAAGAGCCGTGACTACACACGCCCAGACCTGCCTTCCGGGGACAGCCAGCCCTCGCTGGACCAGACCATGGCCGCGGCCTTCGGTGCACCTGGTATAATCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240328171226_0uy3 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240328161152_u77e GCTGCAGTATGCGGACCCCGTGAGCGCCCAGCACGCCAAGCTGTCGCTGGACGG(/C)GCAGAACATCTACAACGCCTGCTGCACGCTGC NGG 10 8 17 8 24 0 100 5 10
    240328104329_6c5m TCCTTCACCTTAAGCCCTGCACTCTCACAGCTGGCTTGGACTGAGTCCATCCCTGGGCCTGCGTTTTTCAAGACAGAAAGATAGCGATGCATCTTAAATTCCACTTCATTTTGTATTTTGAACATCTTAGCTCTTTTCTGTTGCTGCTCC(/t)TTACAGCACAATAATCTGTGAGTTCATGCTGTAATAATTATATTTAAGGCATCTCTACAAGCTAGCTCTGCATGACATTAACATACTCTGTATGACCTCCTGTTATTTAAGTTTTGTGTTTTTTTGCCTGCAGTTCTTGCACTTTCCCCC NGG 10 8 17 8 24 0 100 5 10
    240328103334_84lp CATACAATTTTGCCATCTCAACCGAACTTTGTAGTTAGA(/T)TTCCTTTCTGAGGGCTTTACACCGTACTCCGCTCATCTACGCGGGATCCGGCCTTAGGTTTTCTTTCTTGCATCCATCGCCGCTATCAATCTAACCCC NGG 10 8 17 8 24 0 100 5 10
    240328094323_q1eb CACTAAAGAAGTCTATCTTTAGTACCCAGCTGTGCTTTCTCTTGATGGTTCACTTTGTCCTCCTTCACCTTAAGCCCTGCACTCTCACAGCTGGCTTGGACTGAGTCCATCCCTGGGCCTGCG(/A)TTTTTCAAGACAGAAAGATAGCGATGCATCTTAAATTCCACTTCATTTTGTATTTTGAACATCTTAGCTCTTTTCTGTTGCTGCTCCTTACAGCACAATAATCTGTGAGTTCATGCTGTAATAATTATATTTAAGGCATCTCTACAAGCTAGCTCTGCATGACATTAACATACTCTG NGG 10 8 17 8 24 0 100 5 10