Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240327150113_f6by gaaatagctatcttcttttaaaatgtttgtttcttttcttgctttcaggcattacaatggttgacacagaaatgccgttttggcccattaattttggaattagcccagtggatctgtctgcgatggatgatcatacacattcctttgacataa(/C)aaccatttaccactgttgatttttcaagcatttcttcaccacactatgaagatattcctcttgcaagaactgatcagacaagcattgattataaatatgatatcaagctccaggattgccaaagtatgctttttattttccattatttcttcatgttcacatttgctttatgctcttgaaaatatgctaccaacagtagaaattaga NGG 10 8 17 8 24 0 100 5 10
    240327115746_va6z ccggcgtggtcatgcggccctacgtggaagccaacatctcccacaagtcccacaccaccatcaagtatttcctcaagatgtggagcagcgtgagcgagaccctc(/a)atcttcatcttcctgggtgtctccaccgtggccggccagcactactggaactggacctttgtcatcagcacgctgctcttctgcctcatcgcgagggttttaggtgaggtcccgctgtgaggacaaggtccttggg NGG 10 8 17 8 24 0 100 5 10
    240327102718_s77e TTCTTGTGCTGGGGGCCTCAGAGCTGAACACTGGGGTCTCAGGAGAGCAGAGCAGAGGGGGACAATCCCCTCCCTCACCCTGCTTCCACGCTTCTTTTGATACAGCCCAGGATACGGTTGGCTT(/C)TCTGGGCTGCAAGCACACATTGCTGGCTCATGTCGAGCTTCTTGTCAGCCAACACCCACTAAAGAAGTCTATCTTTAGTACCCAGCTGTGCTTTCTCTTGATGGTTCACTTTGTCCTCCTTCACCTTAAGCCCTGCACTCTCACAGCTGGCTTGGACTGAGTCCATCCCTGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240326211908_jmtc CCAGCGGCAGCACCTCGCGGTAGTGGCTGCGCAGCAGGGAGCGCACGGCTCGGCAGCGGGGAGCGCGCGGCATCGCGGGGGTGGCCGGGGCCAGGGCTTCCCACGTGCGCAGCAGGACGCAGCGCTGCCTGAAACTCGCGCCGCGAGGAGAGGGCGGGGCCGCGGAAAGGAAGGGGAGGGGCTGGGAGGGCCCGGAGGGGGCTGGGCCGGGGACCCGGA(/G)AAGGGGTCGGGACGGGGCGGGGTCCGCGCGGAGGAGGCGGAGCTGGAAGGTGAAGGGGCAGGACGGGTGCCCGGGTCCCCAGTCCCTCCGCCACGTGGGAAGCGCGGTCCTGGGCGTCTGTGCCCGCGAATCCACTGGGAGCCCGGCCTGGCCCCGACAGCGCAGCTGCTCCGGGCGGACCCGGGGG NGG 10 8 17 8 24 0 100 5 10
    240325213023_gowg atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacAC(/T)aTggTgtTcaAtgTttTTCAcgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa NGG 10 8 17 8 24 0 100 5 10
    240325172152_ykoc TAAACACATTTTGTGCCCTCTGAGGGGCGGAGCGGGATTGCTCTGCACTTAGGGTCTCCCTGAGGCGAGGGTCCAGGCTCCACA(/g)GGCCCCATTGACCCTGTGGCACCCAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240325172050_jp7e TTTTTTTTTGTCCCAGTGGGGTCCCAGTGGGGACTGTTTTAGCCAGGGTGGTTGAGGAGCTCAGCTGGAGACACCAGT(/c)GTGTGGCAGATGAGGCAGGAAAGACAAAATTAAGGACATGTTGAGTGCCCTATGAGGAAAATTAAGGATGTATAAACACATTTTGTGCCCTCTGAGGGGCGGAGCGGGAT NGG 10 8 17 8 24 0 100 5 10
    240325171849_jy56 TTTTCCTTTGAGTAACCTTTTTAAAGTTCTTAGAAAGAATCGGTGATAAGATCACAGCAAGCTTTACCACGACTGCCTTTCCCCTTGGTTGGTCTTTCTCACCT(/c)GAAAGCAAACAAGAAGACAGGAGGGACTGCATCCTGGAGCAGGGTCTTCTGATGAAGCAATACTT NGG 10 8 17 8 24 0 100 5 10
    240325171802_040f GCCTTTCCCCTTGGTTGGTCTTTCTCACCT(/c)GAAAGCAAACAAGAAGACAGGAGGGACTGCATCCTGGAGCAGGGTCTTCTGATGAAGCA NGG 10 8 17 8 24 0 100 5 10
    240325171348_mrwy ctggtcgctgccctgctgaaaatggcagatgttactacaaaatgtcactttgtcctgttgctgtgtgtttctttgtcaggtggactgcagtaggtttcccaacgctacagacaagg(/t)aaggcaaagatgtattggtttgcaacaaggacctccgccccatctgtggtaccgatggagtcacttacaccaacgattgcttgc NGG 10 8 17 8 24 0 100 5 10