Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240328093305_mapn GACAATCCCCTCCCTCACCCTGCTTCCACGCTTCTTTTGATACAGCCCAGGATACGGTTGGCTTTCTGGGCTGCAAGCACACATTGCTGGCTCATGTCGAGCTTCTTGTCAGCCAACACCCACTAAAGAAGTCTATCTTTAGTA(/c)CCCAGCTGTGCTTTCTCTTGATGGTTCACTTTGTCCTCCTTCACCTTAAGCCCTGCACTCTCACAGCTGGCTTGGACTGAGTCCATCCCTGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240327230100_c6c9 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacAC(/T)aTggTgtTcaAtgTttTTCAcgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa NGG 10 8 17 8 24 0 100 5 30
    240327230031_amze atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacAC(/T)aTggTgtTcaAtgTttTTCAcgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa NGG 10 8 17 8 24 0 100 5 10
    240327225918_acij atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacAC(/T)aTggTgtTcaAtgTttTTCAcgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa NGG 10 8 17 8 24 0 100 5 10
    240327195706_qg98 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240327151306_t0ir gaaatagctatcttcttttaaaatgtttgtttcttttcttgctttcaggcattacaatggttgacacagaaatgccgttttggcccattaattttggaattagcccagtggatctgtctgcgatggatgatcatacacattcctttgacataaa(/c)accatttaccactgttgatttttcaagcatttcttcaccacactatgaagatattcctcttgcaagaactgatcagacaagcattgattataaatatgatatcaagctccaggattgccaaagtatgctttttattttccattatttcttcatgttcacatttgctttatgctcttgaaaatatgctaccaacagtagaaattaga NGG 10 8 17 8 24 0 100 5 10
    240327151138_j37p gaaatagctatcttcttttaaaatgtttgtttcttttcttgctttcaggcattacaatggttgacacagaaatgccgttttggcccattaattttggaattagcccagtggatctgtctgcgatggatgatcatacacattcctttgacataaaaccatttacca(/c)ctgttgatttttcaagcatttcttcaccacactatgaagatattcctcttgcaagaactgatcagacaagcattgattataaatatgatatcaagctccaggattgccaaagtatgctttttattttccattatttcttcatgttcacatttgctttatgctcttgaaaatatgctaccaacagtagaaattaga NGG 10 8 17 8 24 0 100 5 10
    240327151004_2f5i gaaatagctatcttcttttaaaatgtttgtttcttttcttgctttcaggcattacaatggttgacacagaaatgccgttttggcccattaattttggaattagcccagtggatctgtctgcgatggatgatcatacacattcctttgacataaaaccatttaccactg(/a)ttgatttttcaagcatttcttcaccacactatgaagatattcctcttgcaagaactgatcagacaagcattgattataaatatgatatcaagctccaggattgccaaagtatgctttttattttccattatttcttcatgttcacatttgctttatgctcttgaaaatatgctaccaacagtagaaattaga NGG 10 8 17 8 24 0 100 5 10
    240327150237_yhol gaaatagctatcttcttttaaaatgtttgtttcttttcttgctttcaggcattacaatggttgacacagaaatgccgttttggcccattaattttggaattagcccagtggatctgtctgcgatggatgatcatacacattcctttgacataaaaccatttac(/A)cactgttgatttttcaagcatttcttcaccacactatgaagatattcctcttgcaagaactgatcagacaagcattgattataaatatgatatcaagctccaggattgccaaagtatgctttttattttccattatttcttcatgttcacatttgctttatgctcttgaaaatatgctaccaacagtagaaattaga NGG 10 8 17 8 24 0 100 5 10
    240327150211_6tox gaaatagctatcttcttttaaaatgtttgtttcttttcttgctttcaggcattacaatggttgacacagaaatgccgttttggcccattaattttggaattagcccagtggatctgtctgcgatggatgatcatacacattcctttgacataaaacca(/C)tttaccactgttgatttttcaagcatttcttcaccacactatgaagatattcctcttgcaagaactgatcagacaagcattgattataaatatgatatcaagctccaggattgccaaagtatgctttttattttccattatttcttcatgttcacatttgctttatgctcttgaaaatatgctaccaacagtagaaattaga NGG 10 8 17 8 24 0 100 5 10