Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240322100006_8e7y CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240322095329_ethm GATTATGCTATGATTGTTGTTTGCTTTTCTTACATTTGAAAGTCACAACTGTAACATGGTTGATAACTTGTTTCCTTAATAAGAATTCATTCACTAAAACATCATAATTTTCTCATAAGTCTGGACCAAGAATATTTTCACTATTTGGGAAATAGCTGGAAAATCAGGATTTTTACATTTATTTGACTATTTATGTTACCAGGTCTATAAAAAAAGCAATGATGCCTATATGTACCCTTAACATGTTACATATGAGGTCAAGTTGAGAAGTAACTTACAACTTACATGAAAGAAGAGTGGAG(+atgcatgc)GATCCTGTTGGATTGTTTGGCTGGACACTATTACATTGGAACTGTGTTCACAGAATCAAAGCTGTGTTCCACCTGGGAGGGTGAGTCTTCAGAGTTCATCAGCTAAAAGTTGTCTTGGAACTAATGAAGTTAATAACTCATGGTTCTGCATACCTGCAAAGTATCTGCCCAAATTATTGTGATTCTCTTCAGCACTGGAATGGAATGTGTTCACCATTTGCTAGAAGCCAAGTGTCTTCTTGGAGCTTTACTGTGGCAAGGTAATATTTTTTTTCATTTCAACAAAAGATCAGAGTATTGT NGG 10 8 17 8 24 0 100 5 10
    240321132152_oe4m ACAGGAGAGTGATGGACGCCATCTCTCGTTCCCAGCTTTACCTGGACGACCTCTTTTCTGACTTCTACGACAAACCCCTCAGCATGACTGACATTTCCCTCAAGGAGA(/G)GGACTCACATACGCGTTAACTTACTAAACCACAACATTCCTAAAGGGCCCTGCATCCTCTGCGGAATGGGGAACTTGAAAAGGGAGACGGTCTACGGCTGCTTCCAGTGTTCTGTGGACGGCGTGAAGTACGTGCGTCTTCACGCAGTCCCTTGCTTTGATATCTGGCACAAGAGAATGAAATAAAAGGAAGAAGG NG 10 8 17 8 24 0 100 5 10
    240321131925_kr8h ACAGGAGAGTGATGGACGCCATCTCTCGTTCCCAGCTTTACCTGGACGACCTCTTTTCTGACTTCTACGACAAACCCCTCAGCATGACTGACATTTCCCTCAAGGAGA(/G)GGACTCACATACGCGTTAACTTACTAAACCACAACATTCCTAAAGGGCCCTGCATCCTCTGCGGAATGGGGAACTTGAAAAGGGAGACGGTCTACGGCTGCTTCCAGTGTTCTGTGGACGGCGTGAAGTACGTGCGTCTTCACGCAGTCCCTTGCTTTGATATCTGGCACAAGAGAATGAAATAAAAGGAAGAAGG NGG 10 8 17 8 24 0 100 5 10
    240321111935_dzgs CCTTTTTAAAGTTCTTAGAAAGAATCGGTGATAAGATCACAGCAAGCTTTACCACGACTGCCTTTCCCCTTGGTTGGTCTTTCTCACCT(/C)GAAAGCAAACAAGAAGACAGGAGGGACTGCATCCTGGAGCAGGGTCTTCTGATGAAGCAATACTTAGGAAAGTGCTGCCAGCATCAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240321084133_z3zd CGGTTTCGGGACTACGAAGGGTCGGGGCGGACGGACTCGAGGGCCGG(/C)CCACGTGGAAGGCCGCTCAGGACTTCTGTAGGAGAGGACACCGCCC NGG 10 8 17 8 24 0 100 5 10
    240320164953_crud CCTTTCTGGGAGTTCTCTGCTGCCTCCTGGCTTCTGAGGACCGCCCTGGGCCTGGGAGAATCCCTTCCCCCTCTTCCCTCGTGATCTGCAACTCCAGTC(+aac)TTTCTAGAAGATGGGCGGGAGTCTTCTGGGCAGGCTTAAAGGCTAACCTGGTGTGTGGGCGTTGTCCTGCAGGGGAATTGAACAGGTGTAAAATTGGAGGGACAAGACTTCCCACAGATTTTCGGTTTTGTCGG NGG 10 8 17 8 24 0 100 5 10
    240320164906_6w1m CCTTTCTGGGAGTTCTCTGCTGCCTCCTGGCTTCTGAGGACCGCCCTGGG(+ctt)CCTGGGAGAATCCCTTCCCCCTCTTCCCTCGTGATCTGCAACTCCAGTCTTTCTAGAAGATGGGCGGGAGTCTTCTGGGCAGGCTTAAAGGCTAACCTGGTGTGTGGGCGTTGTCCTGCAGGGGAATTGAACAGGTGTAAAATTGGAGGGACAAGACTTCCCACAGATTTTCGGTTTTGTCGG NGG 10 8 17 8 24 0 100 5 10
    240320164815_oo1z CCTTTCTGGGAGTTCTCTGCTGCCTCCTGGCTTCTGAGGACCGCCCTGG(+ctt)GCCTGGGAGAATCCCTTCCCCCTCTTCCCTCGTGATCTGCAACTCCAGTCTTTCTAGAAGATGGGCGGGAGTCTTCTGGGCAGGCTTAAAGGCTAACCTGGTGTGTGGGCGTTGTCCTGCAGGGGAATTGAACAGGTGTAAAATTGGAGGGACAAGACTTCCCACAGATTTTCGGTTTTGTCGG NGG 10 8 17 8 24 0 100 5 10
    240320164643_h2oa CCTTTCTGGGAGTTCTCTGCTGCCTCCTGGCTTCTGAGGACCG(+aat)CCCTGGGCCTGGGAGAATCCCTTCCCCCTCTTCCCTCGTGATCTGCAACTCCAGTCTTTCTAGAAGATGGGCGGGAGTCTTCTGGGCAGGCTTAAAGGCTAACCTGGTGTGTGGGCGTTGTCCTGCAGGGGAATTGAACAGGTGTAAAATTGGAGGGACAAGACTTCCCACAGATTTTCGGTTTTGTCGG NGG 10 8 17 8 24 0 100 5 10