Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240314025627_62mbu4ji TCAAGCCTCCTGTTTTTAAATTAAGGCAATCCTTGGTGATCAAAG(-GATAGTAGGGG)AAGTGAGTAATAAATATAAGTCAAGCAATCATAGAAATGTGAAAA NGG 10 8 17 8 24 0 100 5 1
    240314025627_62mbt2sq TGTTACCCCAAACAAAATTAGAGCTCTCTTAGCAAGAAAGAAGAT(-GGAAAGGTAGT)AGAGGTAGGCAGCTAATAGTAGCTATCACAAGTATAAAATAAAAT NGG 10 8 17 8 24 0 100 5 1
    240314025627_62mbdxqj AGTCCCCACAGTGGTCCTTTGGGGAATGTAACCTTTTTTATGTGG(-TCTTGATTAAA)TCCCATTTTACTTCCTTGCAGGTTAACAACCATTATTGAGTACCT NGG 10 8 17 8 24 0 100 5 1
    240314025627_62mb9dwk CCGTCGATCCAGAGTCCCTCCACCGTGGTCCTGGAGTCCCCCTCA(-CCCTTGACCCG)GATCCACCCCCACCGTGGAACCAGGGTCGACGCCACCGTGGGCCT NGG 10 8 17 8 24 0 100 5 1
    240314025627_62mb8x76 ACTCTTGCGTTTATCTTTTTGTATAATGAATAGTGAGGGGAGAAT(-GGCAGAGTAAT)TTCTAGAGTAGGCTTAGATGTTGTTACTGTTTATTATACCTTAGA NGG 10 8 17 8 24 0 100 5 1
    240314025627_62mb52nn CCACTTGCTTACTGAGGAATTTCCCCATGAAGAGCCCTCCCCCTG(-TGAAATGTGGC)CTTGCTGGCATTTGAGAACTGACAAAGGGTGGAGTGAATCAGTTG NGG 10 8 17 8 24 0 100 5 1
    240314025627_62mb51vl ACACACTGTTTAATTGTTTGATGGAAGTGACTGGGGAATAGGATT(-GAAGAAGTGAG)AAGAGGGAGGGGCCAAGAAAAAGTGATTTCTGGAGAAGGGCCCTT NGG 10 8 17 8 24 0 100 5 1
    240314025627_62mb1anq TATGCCTTTCAGCTAGTTGATTTGTAATTTTACAGTTTTAAAAAT(-AGAAATGGAAA)CCAATACTTCAAGTTTATGGAAATCTAGAGAGCAAATATGTATAA NGG 10 8 17 8 24 0 100 5 1
    240314025526_i9wox80u AAGAGGCAGATAGAGGAGCATTTGAGTCTGGGTGTTTTTGGAGGC(-TTCTCATACCT)GATTGGTCCCTATAATTATGAGGAAGAGAGGGATGGACATGGTTG NGG 10 8 17 8 24 0 100 5 1
    240314025526_i9wosh45 GCTGGTGAGTGTGCCCAGACCTCCCAGCATCCATGGCCAGCCGGG(-GAGGGTTCGGG)AACACACAGACCCACACACAGGCTCAGGAAAGCATGGGGGTCAGA NGG 10 8 17 8 24 0 100 5 1