Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240314025858_6alnms1w CTGGGGTACAAAAACCCTAGGCCCACAAATCCAGTGAGGTTTCTT(-CCATCATACTG)ACCCTTCTCCTTTGACTCCCTGGGTAGAGTTCAGCCCCTACCCCA NGG 10 8 17 8 24 0 100 5 1
    240314025858_6alnh6l5 TACTTATCAAGTAGTGACAGTAGCTTAAGATACTAGCATCCTGGC(-TGAAGAATGAA)ATCATAAGAATGATGAGTTAGGTTATACTCCCATTAAGTACATCT NGG 10 8 17 8 24 0 100 5 1
    240314025858_6alnf9vk TAGGATGAGAGGCAAGGACAAGCTCAGGGGTTTGGCCTGGATACC(-TGGTTTAGAAT)GAAAGTAGGCTCATTCCCAAGGAAGGAGATGAGCAAGCAGAGCTG NGG 10 8 17 8 24 0 100 5 1
    240314025858_6alne159 TACAAAAGGCTGCATGTTATGTGATTCCACTTCTTTGTCTTTCTG(-GAAAAGGTAAT)ACTATTGAATAAAAATGAGGAGGTAGAGAAGAAGGTACTTCACTT NGG 10 8 17 8 24 0 100 5 1
    240314025858_6aln8qt6 TCCAGACCGGTGGGACTCTGAGCATGCGGGAGGGGTCAAGAAGGC(-AGGGCTGTGGG)TAGGCTCGAGCAGCCAGGGAAGGCTTCCAAGGCAAGGCGGGCTCA NGG 10 8 17 8 24 0 100 5 1
    240314025858_6aln4t5v AGGGGAGTGGGGAGGAAAGGCGGCCATCATGAGGGAGCGTATTCA(-AGGACAGAGCT)TCTCCAGGATGTGCACAGCTGGCCTTCCTTACCTCCCAGGACCTC NGG 10 8 17 8 24 0 100 5 1
    240314025858_6aln3s8a GTAGTTTGATGGGTTTTAGCATATTCACAGAGTGATGGAACCATC(-ACCACAACCTG)AGTTTAAAACATTTCATCACCCCAAAGGGAACTCAGTGTTCATGA NGG 10 8 17 8 24 0 100 5 1
    240314025858_6aln2mpg CCTATGAAATATCAGGTAATGTTCATGAACAGAATACATTAAGGA(-ATCAGAATCAG)TGTAAAGATTTTTAAGTATCCGGGCGCGGTGGCTCACACCTGTAA NGG 10 8 17 8 24 0 100 5 1
    240314025808_ht3hzswl TTACCTAATGGACAGGGAGAACATTTGTTTACTGTTCGCATAGTA(-GGCAGGTCAAG)ATCAATCTGTGATAGAATATCGGAGTTGTATTTGACTTTTTGAAA NGG 10 8 17 8 24 0 100 5 1
    240314025808_ht3hpd67 CAGCGGGTTTGGGGGGATTACCGGCCTGCTGCACGCCCACCCCAG(-CCCCTCAGCTG)CTGCCCCATCCCGAGGCCCCCATCCAGGCTCCCCAGCCCGCAGTC NGG 10 8 17 8 24 0 100 5 1