Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240314222522_61lj CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240314222517_zq3b CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240314221908_m444 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240314091457_uw07 TTGTTTTTTTTCAAGAACATATCCCTGTTCCCACTCGGTAAAAATCCGTTCATTATCATCATTGATATACTCATCGTAAGTTTTTTCATTTATCAATTTTATTCTACCATTCGAAGAACCAGAGGAATCCAGCTATTACTCGGAGTTGCTGTCATTTGGTTCCTTGGAATTTTTGCGAGTACGTTTGAACTAGAACTATTAGATTGGATTATAGAAAGTATTCGTCCTGCACTCGTATTTGCGATTATCGTTTTACTTCAGC(-C)GGAACTTAGAAAGATCACAGCAGATCTTTCTAAAATGAAAATCTTTCAACCGTTTCTTTTAAAACAAATGACAGATTTAGAGGAGATCATAGAGGCCGTAAAGATTATGGCTAAAAATAAAACAGGATCTCTGATTGCGATTGTACGAGAGAATAATTTGAAGGAAATTATAGATCAATCTGTGCAGTTAGACGCGATCATCTCTGCGAGTTTGCTTCTTACTATTTTTAAAAAAAATTCAGCACTTCACGACGGGGCCGTAATTATAGAACAAAATAGAATCGCCTGTGCCGGGGCTTTTCTCCCAATGACTCAGAATTTAGACGACTCAAGAATGGGAGCCAGACACAGAGCCGCACTTGGAATTTCAGAAGAATCGGATTCTATTGTGATTGTTACGTCGGAAGAAACTGGAGAAATTTCTGTATGTTACGACGGAGAAATGACTCATCCTGTCAAACCGATCGAACTTAAAAATCTAGTAAATACGATCCTGAAAAAAAGGGATAATGTTTCTGAAAAATACAATCCAGTTGTAGAAGAAAAGACGGAGCGCTGA NGG 10 8 17 8 24 0 100 5 10
    240314085954_ez88 TCTGGCTTTCCACAGAAAGTAGAATTGTTAAAAAATGTGTGCTGAAAAAGCAAATGGGGCTTGAGATGGCTGAGCACAATTTGCATGGTATTGATTAAGGGG(+ATCTATCT)GTACCTGTGGAGGGTACTATCTGAGAAGCCCATTTAGGATAATTTTGCTAAGTCTTCCAAATGACACATAAATTCCCCATTCTTTACTTTAAAACTTATAG NGG 10 8 17 8 24 0 100 5 30
    240314085836_2nh3 TAGTGTGTATTCCAATAGTATGTGGCCAGCTTATCAAAGTTGTGCACACAATTTGAACTAGCATATTACTTTACTCTTAATTACTGTGCTCACAAAGCCTCG(+ATCCATCC)GATCCTTGGATCTCTTGCATCTGTGACTAACCACAGTGATCATTTCTTTTATTTCTCATACATATATAATACCATTTTTTAATAGATTTTACTCTGAAAAC NGG 10 8 17 8 24 0 100 5 30
    240314085539_nf3r TAGTGTGTATTCCAATAGTATGTGGCCAGCTTATCAAAGTTGTGCACACAATTTGAACTAGCATATTACTTTACTCTTAATTACTGTGCTCACAAAGCCTCG(+ATCCATCC)GATCCTTGGATCTCTTGCATCTGTGACTAACCACAGTGATCATTTCTTTTATTTCTCATACATATATAATACCATTTTTTAATAGATTTTACTCTGAAAAC NGG 10 8 17 8 24 0 100 5 10
    240314085443_t9qq TAGTGTGTATTCCAATAGTATGTGGCCAGCTTATCAAAGTTGTGCACACAATTTGAACTAGCATATTACTTTACTCTTAATTACTGTGCTCACAAAGCCTCG(+ATCCATCC)GATCCTTGGATCTCTTGCATCTGTGACTAACCACAGTGATCATTTCTTTTATTTCTCATACATATATAATACCATTTTTTAATAGATTTTACTCTGAAAAC NGG 10 8 17 8 24 0 100 5 10
    240314025948_11trr0tz CCTGCCCCCTGCCCCTTGTTCCTGATGTGTCAGTGTCTAGGGCTG(-TCCCACCCCCA)CCCTCTCTGGTCTTTCATAGAAAGGGGAACAGCCTCAGGGCCACT NGG 10 8 17 8 24 0 100 5 1
    240314025948_11trl0ww CTAGAGGTGAGAGCAGCTCACCCCACTACCAGACTCTGTGTTTAG(-GGTGGTGACCT)GAAGAAGGAAGAGAGCGAAAGAAGGGAAGGACCATCTTTCCCTCT NGG 10 8 17 8 24 0 100 5 1