Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240316051510_umr7 TTATCAAAGAACTCACCGCCCTGGGGCGGCCAGATATCCTTGTCA(/T)TGTGTGGGGGCGTGATTCCACCACAGGTATTTTTCCTTCTCTGTACTCTCCTGCAC NG 15 8 17 8 25 0 100 5 10
    240316050746_wmcw CTCCAGAAAGTTCACAACGGCATGGATGACCTCATTTTAGATT(/G)GGCACAATATTTTAGATGGACTGAGGACCCAGAGACTGACCTTGAAG NG 15 8 17 8 35 0 100 5 20
    240316050742_2jle CTCCAGAAAGTTCACAACGGCATGGATGACCTCATTTTAGATT(/G)GGCACAATATTTTAGATGGACTGAGGACCCAGAGACTGACCTTGAAG NG 15 8 17 8 35 0 100 5 20
    240316050736_5bi4 CTCCAGAAAGTTCACAACGGCATGGATGACCTCATTTTAGATT(/G)GGCACAATATTTTAGATGGACTGAGGACCCAGAGACTGACCTTGAAG NG 15 8 17 8 35 0 100 5 10
    240316050729_729w CTCCAGAAAGTTCACAACGGCATGGATGACCTCATTTTAGATT(/G)GGCACAATATTTTAGATGGACTGAGGACCCAGAGACTGACCTTGAAG NG 15 8 17 8 35 0 100 5 10
    240316050701_dars CTCCAGAAAGTTCACAACGGCATGGATGACCTCATTTTAGATT(/G)GGCACAATATTTTAGATGGACTGAGGACCCAGAGACTGACCTTGAAG NGG 10 8 17 8 24 0 100 5 10
    240316050535_hc66 CTCCAGAAAGTTCACAACGGCATGGATGACCTCATTTTAGATT(/G)GGCACAATATTTTAGATGGACTGAGGACCCAGAGACTGACCTTGAAG NGG 10 8 17 8 24 0 100 5 10
    240315160924_dzm8 CATTCCAGTGTGAAACCTGTCAGAGAAAGTTTTCACGTTCAGACCACCTTAAGACCCACACCCGGACACATACAGGT(/G)AAAACAAGTGCGTAAACCTTTTCATTTTTTTCATGATTCGCTCCTCTCTTTCCACTCTTTGATTTAAAGTTGAATTTTTAGAGGTCACTTTTTTATCTCAGCTTGTACTGGGAAGTTATTAAAAAATATTTGCAGTGTTTTTTTTTATTAT NGG 10 8 17 8 24 0 100 5 10
    240315093814_cqyo CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240314222525_7p33 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10