Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240403172523_89zl tccctgctcttgatggggaggatgctagggctacgtgttcgttacatgcagactccaggtgtacgttgccgagctaaccctgcaggagaggagaactagagagtgtgtcctggaggtatttctgagggattgttcaaaattactcttatttctgctgggttgtgaaactctaggcagtgatgaccttactacctttaaggtcacagaaaccagcacagtgcctggcacatggttggtgatctgagtgccgggttgtttataaaggacagaagattcggcagaactaagcaggcgtcagaggagttggtgggtgtgagtgcccctgtccctgcacttcgggtggctgctggtcctccgggtcctgctgtgtggttagacggcttccgggcagcctggtctggccagcactcaccctgccctctctgccttttctcccccagGGTATTTGGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCG(/A)CTTGGCGAGGCAGAGACTGCTGGGCCGGTCATGGAGCGTGCCAGTCATCCGCCACCTCTTCGCTCCGCTGAAGGAGTATTTTGCGTGTGTGTAAGGGACATGGGGGCAAACTGAGGTAGCGACACAAAGTTAAACAAACAAACAAAAAACACAAAACATAATAAAACACCAAGAACATGAGGATGGAGAGAAGTATCAGCACCCAGAAGAGAAAAAGGAATTTAAAACAAAAACCACAGAGGCGGAAATACCGGAGGGCTTTGCCTTGCGAAAAGGGTTGGACATCATCTCCTGATTTTTCAATGTTATTCTTCAGTCCTATTTAAAAACAAAACCAAGCTCCCTTCCCTTCCTCCCCCTTCCCTTTTTTTTCGGTCAGACCTTTTATTTTCTACTCTTTTCAGAGGGGTTTTCTGTTTGTTTGGGTTTTGTTTCTTGCTGTGACTGAAACAAGAAGGTTATTGCAGCAAAAATCAGTAACAAAAAATAGTAACAATACCTTGCAGAGGAAAGGTGGGAGAGAGGAAAAAAGGAAATTCTATAGAAATCTATATATTGGGTTGTTTTTTTTTTTGTTTTTTGTTTTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240403003757_jidi CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240402090752_wyrd CTTCTGGACCACGGCATACCGACGCACGCAGTACGTGTTCTGGGGTTTGATGATGCTGGCGATGACCTCCGTGAGCCTGTCCTGGGGGATGGTGTCGTACA(/G)CGCCCGTCACATCCACCTGTGTGAGTGGAGGCGAGGAGACTGACAGTGGCCACGCAGAAACTCAGACATCACCTCTGCCCTCAGGGCCTGGCCTGGCGGT NG 10 8 17 8 24 0 100 5 10
    240402090740_gy63 CTTCTGGACCACGGCATACCGACGCACGCAGTACGTGTTCTGGGGTTTGATGATGCTGGCGATGACCTCCGTGAGCCTGTCCTGGGGGATGGTGTCGTACA(/G)CGCCCGTCACATCCACCTGTGTGAGTGGAGGCGAGGAGACTGACAGTGGCCACGCAGAAACTCAGACATCACCTCTGCCCTCAGGGCCTGGCCTGGCGGT NGG 10 8 17 8 24 0 100 5 10
    240402090255_vwvz CTTCTGGACCACGGCATACCGACGCACGCAGTACGTGTTCTGGGGTTTGATGATGCTGGCGATGACCTCCGTGAGCCTGTCCTGGGGGATGGTGTCGTAC(/G)CGCCCGTCACATCCACCTGTGTGAGTGGAGGCGAGGAGACTGACAGTGGCCACGCAGAAACTCAGACATCACCTCTGCCCTCAGGGCCTGGCCTGGCGGT NG 10 8 17 8 24 0 100 5 10
    240402090235_5hsr CTTCTGGACCACGGCATACCGACGCACGCAGTACGTGTTCTGGGGTTTGATGATGCTGGCGATGACCTCCGTGAGCCTGTCCTGGGGGATGGTGTCGTAC(/G)CGCCCGTCACATCCACCTGTGTGAGTGGAGGCGAGGAGACTGACAGTGGCCACGCAGAAACTCAGACATCACCTCTGCCCTCAGGGCCTGGCCTGGCGGT NGG 10 8 17 8 24 0 100 5 10
    240402024152_xvb7 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240402024055_o33c CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240401061536_dws7 TGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCG(/A)CTTGGCGAGGCAGAGACTGCTGGGCCGGTCATGGAGCGTGCCAGTCATCCGCCAC NGG 10 8 17 8 24 0 100 5 10
    240401061355_ap4y CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10