Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240314020740_ooo4gmgk ATCATAGGATTAGACTTGGTAGATACAAAGGAATTTTAAGGTAGA(-CAAGGAATTTA)CATTTTTGTTTGAAGAACTGGCTTATATTTTTAAAACAAATTGGA NGG 10 8 17 8 24 0 100 5 1
    240314020740_ooo4fvzb CTTGGTGATTCTGATTTCCTGACTTGGAGTGGATTAAAGCAGGAA(-ATTAAGAGGGA)GTCAGGCTTTTTTTTTTTTTTTTTAGGCAGTATCTGTCTCTCTTG NGG 10 8 17 8 24 0 100 5 1
    240314020740_ooo4fte0 TTCAGAACAGTGAGGAACTGATATTTAGTCATTTCTGTGCACCCC(-TGGTCACGCAG)TTGCGCCCCCCCCCCCTTTTTTTTCCTTAACTTTGTTGTTTTTTG NGG 10 8 17 8 24 0 100 5 1
    240314020740_ooo4daj2 AGGTTGTCTGTTCCAAGGTATGCATTGTATTCATCTGTGTAATGG(-ATTTAGGTGAA)GGTAGTCATGTAGTTGCTTTGAGATTTTATTTTTTTGCTAAAGTT NGG 10 8 17 8 24 0 100 5 1
    240314020740_ooo4d7c0 CATCATCAACAGGAAGGTCCTAGGCAGCCGGGTGTTTGGGAATAA(-GGTAAACAATA)ACTTCCTAAGGGCTCCTAAGCCGCTACTAGACCCAGGGGTCAGGG NGG 10 8 17 8 24 0 100 5 1
    240314020740_ooo4d4r3 ATGACACTGTGTAAGATTTTATTCTGAGGTTCTGACACCTGCATA(-TGGTGGATGAA)GGCGCTCAAGCCTTTGAGAATTGAAGGCAGTGCTTAAGTGGATGA NGG 10 8 17 8 24 0 100 5 1
    240314020740_ooo4cbzg GAAGCTTGGGGTGTTTCAAAGAGATTCTTTTCGTGTTTTTGTGGT(-ATTTGGGTTTA)AGGGAGTGTACTGTTTGTTGCTGCAAACTACTGGCTTTGTTTTCA NGG 10 8 17 8 24 0 100 5 1
    240314020740_ooo4bg0j GGAGCACCAGCCCCTTTGTCCCACCACTCTGAGGAAAGGCACAGG(-GACAGCTGGTC)CAGCTTACAAAGGCCTCTTTCAGGGACCAGTCTGTCCAGCTCTGC NGG 10 8 17 8 24 0 100 5 1
    240314020740_ooo4ahdy TTGATTGGAGTGTGTTGTACATGTGATTGAATGTTATAGAGGCAG(-AAAAAAGATGA)AGAGCTCTAAATTTCTGCCCTCTGGTAAGCTTTTTAGAAAAATAT NGG 10 8 17 8 24 0 100 5 1
    240314020740_ooo4abrv GTGAGTGTCCCTGTACCCACCCCACAGCACTGGCAGGGCCCACAC(-CACCACGGGAC)AGGCCCAGTCCCCGCCATGCAACCTCTGCCTGGGCCAAGGAGAGG NGG 10 8 17 8 24 0 100 5 1