Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240314020611_20x6nvt1 GAATGGTGGGTTGGATGCTCTGGAAATTAAGAGGGACCATCTCAT(-GGACATGATTC)CCTGCGGGTAACCCCAACACAGAGGTGGAGAAACGCGGTGGTAAC NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6nsgk TCAGAGTTATAAGGAATTGTTTTCCTTAGAAAACTTAACATAAAG(-GGGAAAGTAAA)AATTCTGTTGTCCGAACACTGCCTTTGACCTTATACTACGAATTT NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6n884 AGTCCCCACAGTGGTCCTTTGGGGAATGTAACCTTTTTTATGTGG(-TCTTGATTAAA)TCCCATTTTACTTCCTTGCAGGTTAACAACCATTATTGAGTACCT NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6n2za AAATAAAATTTAATATGAGAGGGCAGACCTTGAAAAGGTGGTGGG(-AGGTGGTCCTG)TGGTTTTTTTTTTTTTTTTAAATCACATGAAATATTTCAAAAATA NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6mvid TTACAACCACGGGAATAAAAACAGAGAAGATGCCTACCTTCATGG(-AATTTACAGTC)TTATTTTGTTTTATAGAAATCATGTGTCCTAGTAATCAAATGGGG NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6mhiz ACCGTTCCTTTTAGAAATTTGTGTATTTGTTGTGCAGATGACAAG(-CAACCAGCCAT)ATCTTTTCGTTTGTTTCCTGCGTTCCCTTTAACCATGAGTCCCTA NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6m1sl GCGGCAGCTCTGAGCCCAGGCGCTTTCCTTAGACTTGCAGCGATG(-CACGGATTGCA)TGGGTGGGGGGCGGGGTGGGGAATGAATGCCGGTGCATGCACTTT NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6lz7s CAATATAGTAGTAGCTGCATATGATTGAAAATTTACTAGGTCACT(-AGTGCACCAAA)AATTTTATTCACAGCCTCCAGGCATCTTTTGAAATGTGCAACAAA NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6lx86 GAAGCTTGGGGTGTTTCAAAGAGATTCTTTTCGTGTTTTTGTGGT(-ATTTGGGTTTA)AGGGAGTGTACTGTTTGTTGCTGCAAACTACTGGCTTTGTTTTCA NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6lwl7 GGGCTCTCACGCAACTTTGGGGAGCAACCACAGGCTTTTCTTTTG(-GTGTGTTATAG)AGACCCTCATCCATCTGGCATGGTTAGGAAATGAAAAAATTGGTT NGG 10 8 17 8 24 0 100 5 1