Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240314020611_20x69gi2 GCGGCCGCGCCCCGCAGCTCTGTAGGCCCTGAAGCTCGGGCTAGG(-AGAGTGGTCCG)CAGGTGGTACCGCACGGGAAGGGACGGATGCGCGGCCGAGCTGGC NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x69fl9 TCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCCGGGCCG(-ATCTTATTTTT)TTGTGTCCACAGCATGGATTTCCCTGGTGATCCACCTGCCCCTGC NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x69b8y TTCTAATCACTGCCTGTAATTGCAGAGCAGGCCAAGCCACTAATG(-ACGGAGCAGGA)TAAATCACCAACCACCTTGTTTAAAAGGGTCTCCAAGTCTGAAAC NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x69ady GGAGCTCCATTTGGTATGTTTGTTTTGTTTTCCGGCCAAAAAATA(-ATGTGCGTGTT)GCCTGGTGATAGCATGACCTTTTATTGCAGTTTCAAGTTGTCTAC NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x693d4 GATAAGGACAGGCCTGGGGAATGGGGATGATATGTGTGGCATGTG(-AGGTAAATTGG)GTAAATGACAGACTATTTTATATTTGTGACTTACGCGTCTTTTCA NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6909i TTGACAAACCCAGACACGCCGGGTCCAAGCACCCTTCTCCCATTC(-CCCAAAGTCGT)GCCCATCCTCCTGCCCTGCCCTGCTGCACCCAGTTTTCTCCAAAG NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x68x28 CCATGTTTTCGCTGTAACTGCTCTGCTACCTTGGGAACCTGGCAC(-CTTGCATGACT)TTGTCCACCTGGCATCCCACTATATGCACTTGTACTTAGGCTGAT NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x68htn TGTTACCCCAAACAAAATTAGAGCTCTCTTAGCAAGAAAGAAGAT(-GGAAAGGTAGT)AGAGGTAGGCAGCTAATAGTAGCTATCACAAGTATAAAATAAAAT NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x68hh1 TATGCCTTTCAGCTAGTTGATTTGTAATTTTACAGTTTTAAAAAT(-AGAAATGGAAA)CCAATACTTCAAGTTTATGGAAATCTAGAGAGCAAATATGTATAA NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x67qtu AGGGGAGTGGGGAGGAAAGGCGGCCATCATGAGGGAGCGTATTCA(-AGGACAGAGCT)TCTCCAGGATGTGCACAGCTGGCCTTCCTTACCTCCCAGGACCTC NGG 10 8 17 8 24 0 100 5 1