Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240314020611_20x6gxfx TCGGGTAAGGGGTTACATCCGGGAAACTCGTCAGGGAAATGGCGG(-GAGATGCTGAG)GGTGCGGGGCCTTTGGAATTCCTTGTTCCTTGCCCTCACCTTTAC NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6guf8 CCACTTGCTTACTGAGGAATTTCCCCATGAAGAGCCCTCCCCCTG(-TGAAATGTGGC)CTTGCTGGCATTTGAGAACTGACAAAGGGTGGAGTGAATCAGTTG NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6gi32 TCTCCCGTTTTGTTTTAGTATTAGTGGAAGAAACTGGATTGGATA(-TCAGATCTGTT)TGAAAATGTATTTTCACATGGATCATTTTTTATTAATCTTTGAAT NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6gave GAATATAGTAGGCATATGTATAATCTAATGTTAAAATAAGTTATA(-TAATGGGTAAT)AATTTTTAGATTTCTTAGATAAGTTACACATTCCCAGGATGAGGT NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6frmj GGAGGCGCTAGACAAGGTAGGCACAAGCCTGTCCCAGAGTGGGGA(-CGGAGGGAAGG)CCAGGCGGCCAGAGGGTGGGGTCAGGGCGGACAGAAACAAGTGTA NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6fmgd ACACACTGTTTAATTGTTTGATGGAAGTGACTGGGGAATAGGATT(-GAAGAAGTGAG)AAGAGGGAGGGGCCAAGAAAAAGTGATTTCTGGAGAAGGGCCCTT NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6fizy AAGAGGCAGATAGAGGAGCATTTGAGTCTGGGTGTTTTTGGAGGC(-TTCTCATACCT)GATTGGTCCCTATAATTATGAGGAAGAGAGGGATGGACATGGTTG NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6egh3 CAGGCGGCACCAGGCGGAGCCGCGAGGCCTGGAGAGTCCGAGTGG(-GGAGGGGTCCG)CGCGCCCAGCGTGCGAAACAAACCGGGGACCCGGATCCCCGTTGC NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6eeyt CAGCGGGTTTGGGGGGATTACCGGCCTGCTGCACGCCCACCCCAG(-CCCCTCAGCTG)CTGCCCCATCCCGAGGCCCCCATCCAGGCTCCCCAGCCCGCAGTC NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x6dy9a TCAAGGACTTCTTGGCAGGTGGGGCCCTAGAGCTCAGATGTGGAG(-GAAGAGGGAGG)AGCTGGGTGTGGGCAGGAGCAGAGGGTCCTAGGCTGAGGGAGAGC NGG 10 8 17 8 24 0 100 5 1