Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240314020611_20x61ikh ACTCTTGCGTTTATCTTTTTGTATAATGAATAGTGAGGGGAGAAT(-GGCAGAGTAAT)TTCTAGAGTAGGCTTAGATGTTGTTACTGTTTATTATACCTTAGA NGG 10 8 17 8 24 0 100 5 1
    240314020611_20x60uus TTGGGAAGTGTGGTTTTGACAAGTCAGAATTCTTTCAAGACTTAA(-GGCAGCTGCTG)TAGGCTATCTTTGAGGATAGTCATGGAGCAGTAGTGTTTGGAATG NGG 10 8 17 8 24 0 100 5 1
    240314020516_769nypfp TGAACGGCTCCCAGAACCTTGTTCGTGTGTATGTGTGTAGACAAA(-ATGGCGCCGCG)GCCTTCTTCTGCGCCCGCCATCATGGCTGCCTGCTGTAGGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240314020516_769nuod7 GAAGCCTCTTAATCTTGGAGTGACTTTGGAGATTTTAATGGCAGT(-AGGTAATTCCT)TTACTGTTACTGTTGGCAGAATCTAAAGTTGAAGGTCCCTTCCAC NGG 10 8 17 8 24 0 100 5 10
    240314020516_769nsykn CACCACCCTGCCACCAGGGACCATCCAATACCTGACTGACACCTC(-CAAGTGGGTAC)CATCCTGCCTCCATTGCACACACCCACCTTCCCGCCCCACCCTGT NGG 10 8 17 8 24 0 100 5 10
    240314020516_769nnnez TCGGGTAAGGGGTTACATCCGGGAAACTCGTCAGGGAAATGGCGG(-GAGATGCTGAG)GGTGCGGGGCCTTTGGAATTCCTTGTTCCTTGCCCTCACCTTTAC NGG 10 8 17 8 24 0 100 5 10
    240314020516_769nn19i CCCGAGCCCGGGGGAGGAGGGGAGTGCCCGTGTCCGTGTTGGGGT(-TTGAGGGGTCG)GGAGCGGCGTTGGTGGCGCGAGGTGTGAGGCTGCCCGCCGACGGA NGG 10 8 17 8 24 0 100 5 10
    240314020516_769nlsxn CTGGACAGTATAAAGACAACCCAGTATCAGATAACTGGGATGGAC(-CCATTTGAAGG)ATAGTCTGGTTGTGGATTGGTAGCACGTGGTAGTTTCTGTGCTAA NGG 10 8 17 8 24 0 100 5 10
    240314020516_769nkd5m CAATATAGTAGTAGCTGCATATGATTGAAAATTTACTAGGTCACT(-AGTGCACCAAA)AATTTTATTCACAGCCTCCAGGCATCTTTTGAAATGTGCAACAAA NGG 10 8 17 8 24 0 100 5 10
    240314020516_769nc2gv TTCTAATCACTGCCTGTAATTGCAGAGCAGGCCAAGCCACTAATG(-ACGGAGCAGGA)TAAATCACCAACCACCTTGTTTAAAAGGGTCTCCAAGTCTGAAAC NGG 10 8 17 8 24 0 100 5 10